Mechanisms of mitochondrial apoptosis in combined trauma of the chest and hips and justification of the usage of antioxidants in experiment

  • M.I. Marushchak
  • M.M. Khudobiak
  • G.G. Gabor
  • O.P. Mialiuk
Keywords: combined trauma, free oxygen radicals, transmembrane potential, apoptosis, correction.

Abstract

The aim of our study was to determine the features of apoptosis when there is a combined trauma of the chest and hips and substantiate the efficacy of antioxidants in this condition. Statistically significant hyperproduction of reactive oxygen leukocytes within 1 day of the experiment, which causes disruption of mitochondrial membrane by bioelectrical activity, characterized by dynamic growth in the percentage of leukocyte cell suspensions with low transmembrane potential of a maximum of 7 days (2.6 times higher than control rate, р≤0.001) in posttraumatic period after undergoing combined trauma of the chest and both thighs. It was also dynamic, statistically significant increase in the percentage of white blood cells with signs of apoptosis since day 1 of the experiment with the highest values in the period of 7-14 days. Statistically significant average relationship between reactive oxygen and severity of apoptosis in the first 7 days and 28 days (r≤0,05) and true interdependence between the level of the transmembrane potential and apoptosis through 3 (rxy=0.63) and 7 days (rxy=0.70).

The usage of antioxidants in posttraumatic period of combined trauma of the chest and both thighs have the positive effect of decreased percentage of free oxygen radicals (Emoxipin action is evident after 7 and 28 days, Mexicor – starting from 3 days with a maximum action after 7 days). The percentage of cells with low leukocyte suspension transmembrane potential and FITC Annexin V-positive cells leukocyte reduced suspension of the application of the two adjustment factors, but a positive impact Mexicor respect Emoxipin is significantly higher.

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References

1. Golikov, A. P., Davydov, B. V. & Rudnev, D. (2005). Vliyanie meksikora na okislitelnyj stress pri ostrom infarkte miokarda. Kardiologiya, 7, 21–26. [in Russian].
2. Zadnipryanyj, I. V. & Sataeva, T. P. (2013). Primenenie antigipoksantov v korrekcii antenatalnoj gipoksii s pozicij ee morfo-funcionalnyh osobennostej (obzor literatury). Zhurn. klin. ta eksperym. med. doslidzh., 1, 13–21. [in Russian].
3. Saushev, A. S., Boyarinov, G. A. & Usanova, A. A. (2010). Medikamentoznaya profilaktika reperfuzionnyh povrezhdenij pri tromboliticheskoj terapii infarkta miokarda. Obshaya reanimatologiya, 1, 64–68. [in Russian].
4. Medyanka, Yu. S. (2011). Vliyanie meksikora na lipidnyj spektr krovi u bolnyh stabilnoj stenokardiej napryazheniya. Klinichna ta eksperymentalna patolohiia, 10, 2(36), 65–68. [in Russian].
5. Pavlov, S. V. (2012). Vplyv tiolovykh antyoksydantiv na vmist stres-bilka HSP70 u hipokampi monholskykh pishchanok z hostroiu ishemiieiu holovnoho mozku. Farmakolohiia ta likar. toksykolohiia, 1(26), 15–18. [in Ukrainian].
6. Kozak, D. V. (2011). Patent 63997 Ukraina, MPK G 09 B 23/28. Kyiv: Derzhavne patentne vidomstvo Ukrainy. [in Ukrainian].
7. Kaplya, A. A., Sorokina, L. V. & Hizhnyak, S. V. (2015). Pereprogrammirovanie energeticheskogo metabolizma mitohondrij v zlokachestvennyh novoobrazovaniyah. Ukrainskyi biokhimichnyi zhurnal, 87(6), 19–35. [in Russian].
8. Rebrova, O. Yu. (2003). Statisticheskij analiz medicinskih dannyh. Primenenie paketa prikladnyh programm STATISTICA. Moskva: Mediasfera. [in Russian].
9. Stacenko, M. E., Turkina, S. V. & Kosivcova, M. A. (2013). Vozmozhnosti Meksikora pri ego ispolzovanii v sostave kombinirovannoj terapii u bolnyh ishemicheskoj boleznyu serdca i saharnym diabetom 2-go tipa. Klinicheskaya medicina, 5, 59–64. [in Russian].
10. Tretyakova, O. S. (2003). Kardiozashita ishemizirovannogo miokarda novorozhdennyh v usloviyah gipoksii: sovremennye napravleniya. Liky Ukrainy, 11, 5–10. [in Russian].
11. Filchenkov, A. A. (2013). Reaktivatory apoptoza kak preparaty celevoj protivoopuholevoj terapii. Biomedicinskaya himiya, 59(2), 119–143. [in Russian].
12. Khudobiak, M. M. (2016). Shliakhy korektsii vilnoradykalnoho okysnennia v rannii posttravmatychnyi period pislia poiednanoi travmy hrudnoi klitky i stehon. Medychna ta klinichna khimiia, 18(1), 108–111. [in Ukrainian].
13. Shirokova, A. V. (2007). Apoptoz. Signalnye puti i izmenenie ionnogo i vodnogo balansa kletki. Citologiya, 49, 5, 385–394. [in Russian].
14. Mihin, V. P., Pokrovskij, M. V. & Gureev, V. V. (2011). Effektivnost otechestvennogo miokardialnogo citoprotektora i ego farmakodinamika pri ostroj ishemii miokarda: klinicheskie rezultaty i eksperimentalnye modeli. Rossijskij kardiologicheskij zhurnal, 2, 37–42. [in Russian].
15. Maianski, N. A., Maianski, A. N., Kuijpers, T. W. & Roos, D. (2004). Apoptosis of neutrophils. Acta Haematol., 111(1–2), 56–66.
16. Baraboy, V. A. (2006). Bioantioxidants. Kiev: Kniga plyus.
17. Li, W., Liu, H. & Zhou, J. S. (2012). Caveolin-1 Inhibits Expression of Antioxidant Enzymes through Direct Interaction with Nuclear Erythroid 2 p45-related Factor-2 (Nrf2). J. Biol. Chem., 287(25), 20922–20930.
18. Gogvadze V., Orrenius, S. & Zhivotovsky, B. (2008). Mitochondria in cancer cells: what is so special about them? Trends Cell Biology, 18(4), 165–173.
19. Hamanaka, R. & Chandel, S. (2010). Mitochondrial reactive oxygen species regulate cellular signalling and dictate biological outcomes. Trends Biochem. Sci., 35(9), 505–513.
20. MRC/RCOG Working Party on cervical cerclage. (2003). Final report of the Medical Research Council. Royal College of Obstetricians and Gynaecologists multicentre randomised trial of cervical cerclage. British Journal of Obstetrics and Gynaecology, 100, 516–523.
21. Weir, E. K. & Reever, J. T. (Ed.). (2009). Pulmonary Vascular Physiology and Pathophysiology. New York: Marcel Dekker.
22. Fossati, G., Moulding, D. A. & Spiller, D. G. (2003). The mitochondrial network of human neutrophils: role in chemotaxis, phagocytosis, respiratory burst activation, and commitment to apoptosis. J. Immunol., 170(4), 1964–1972.
23. Marushchak, M. I., Khudobiak, M. M., Antonyshyn, I. V. & Mialiuk, O. P. (2017). Violation of cells energy supply as one of the mechanisms of combined trauma of the chest and both thighs in rats and ways of its correction. Journal of Education, Health and Sport., 7(1), 272–283.
Published
2017-09-29
How to Cite
Marushchak, M., Khudobiak, M., Gabor, G., & Mialiuk, O. (2017). Mechanisms of mitochondrial apoptosis in combined trauma of the chest and hips and justification of the usage of antioxidants in experiment. Reports of Vinnytsia National Medical University, 21(1(2), 204-210. Retrieved from https://reports-vnmedical.com.ua/index.php/journal/article/view/109