Study of antioxidant and anticytolytic activity of extract from Prunus domestica leaves


Keywords: Prunus domestica leaves, lipid peroxidation, antioxidant and anticytolytic activity.

Abstract

Annotation. One of the actual problems of current science is search and creation on the basis of plant poliphenolics new medical products for pharmacocorrection of functional disorders of the liver. The aim of the work was to study the antioxidant and anticytolytic properties of the extract from Prunus domestica leaves on the tissue of the model liver pathology and to determine its most therapeutically active dose. The subject of investigations was a dry extract obtained from the leaf of European plum as well as the drugs of comparison – silibor and α-tocopherol. The tests were carried out on 39 animals divided into experimental groups: intact control, control pathology, the group of animals that were given the investigated extract at the appropriate doses and the group of animals that were given the drugs of the comparison. Functional disorders of the liver were caused by intra-gastrical injection of 50%-olium solution of hepatotoxin – tetrachloromethane. Evaluation of the efficacy of administration of extract from European plum leaf was carried out according to the amount of TBC-active products in liver homogenate and the activity of cytolytic enzyme alanine transaminas in blood serum. Biochemical and statistical (Statistica 6.0 program, Student’s t-criterion, Mann-Whitney test) analysis methods were used in the investigations. The accepted significance level was p≤0.05. As a result of experimental studies it was found that the extract from leaves Prunus domestica at all doses tested showed a different ability to hinder the processes of lipid peroxidation in vitro conditions, and at doses of 1.0 and 2.0 mg/g did not come from the antioxidant properties of α-tocopherol. The most therapeutically effective dose of the extract from leaves Prunus domestica was found to be 25 mg/kg, which maximally halved the formation of lipid peroxidation products on the body of acute tetrachloromethane hepatitis. The extract showed different antioxidant properties exceeding the activity of silibor by 2.3 times and did not match the drug for anticytolytic activity, reducing the activity of the enzyme alanine transaminas by 56.0% compared to the control pathology.

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Published
2022-03-28
How to Cite
Seniuk, I. V., Kravchenko, V. M., & Tkachenko, O. V. (2022). Study of antioxidant and anticytolytic activity of extract from Prunus domestica leaves. Reports of Vinnytsia National Medical University, 26(1), 12-16. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2022-26(1)-02