Evaluation of antibacterial and antiviral barrier function of the conceptual model of a pneumatic helmet

Keywords: pneumatic helmet, antibacterial protection, antiviral protection, aid for respiratory protection, bacteriophages.


Annotation. The global pandemic has necessitated the development of new respiratory protective equipment that seems to combine reliability and ergonomics. The article tests an innovative conceptual model of a pneumatic helmet with a laminar air supply system for its permeability to bacteria Micrococcus luteus and antistaphylococcal bacteriophages. The latter were fed in the form of an aerosol to the external filter of the working device with subsequent titration and seeding. Evaluation of bacterial infection is performed by counting the units that form colonies by multiplying the above indicator by the degree of dilution. The presence of bacteriophages and their number was determined by the Grace method with the calculation of plaque-forming units in the culture of Staphylococcus aureus. Statistical processing of the results was performed on a personal computer Intel Core i3 using one- and multi-factor analysis of variance (licensed software packages Microsoft Office 2016 and Statistica 10). In the full and partial configuration of the device (if there is only an external or internal filter), the penetration of microorganisms into the internal space is not detected. The results of the study confirmed the reliability of the protection system for 6 hours of continuous operation, which makes the conceptual model of a pneumatic helmet a more ergonomic alternative to the usual means of respiratory protection.


[1] Chang, R., Kwok, P., Khanal, D., Morales, S., Kutter, E., Li, J., & Chan, H. K. (2020). Inhalable bacteriophage powders: Glass transition temperature and bioactivity stabilization. Bioengineering & translational medicine, 5(2), e10159. https://doi.org/10.1002/btm2.10159

[2] Cheberiachko, S., Deryugin О., Mirnenko, V., & Borodina, N. (2020). Selection of effective filter respirators. Challenges and opportunities. Journal of Scientific Papers “Social Development and Security”, 10(4), 23-41. https://doi.org/10.33445/sds.2020.10.4.3

[3] Danilevich, Yu. O., Mazur, L. P., & Yastremskaya, S. O. (2020). Особливості раціонального використання засобів індивідуального захисту при роботі з пацієнтами з гострою респіраторною хворобою COVID-19 на амбулаторному етапі [Features of rational use of personal protective equipment when working with patients with acute respiratory disease COVID-19 at the outpatient stage]. Медсестринство – Nursing, 2, 5-8. https://doi.org/10.11603/2411-1597.2020.2.11228

[4] Dolgikh, W. O. (2020). Маска: защитное средство или модный тренд? [Mask: Protective or Fashion Trend?]. Инновации и дизайн, сборник трудов международной научно-практической конференции – Innovation and design, collection of proceedings of the international scientific and practical conference, 1, 144-146. doi: 10.24412/cl-35915-2020-1-144-146

[5] DSTU EN 149: 2017. Засоби індивідуального захисту органів дихання. Фільтрувальні півмаски для захисту від аерозолів. Вимоги, випробування, маркування (EN 149:2001+A1:2009, IDT) Чинний від 2018-02-01 [Respiratory protection. Filter half masks for protection against aerosols. Requirements, tests, markings (EN 149: 2001 + A1: 2009, IDT). Valid from 2018-02-01]. Київ: Держспоживстандарт України – Kyiv: Derzhspozhyvstandart of Ukraine. URL: http://online.budstandart.com/ua/catalog/doc-page?id_doc=75012

[6] Li, J., Leavey, A., Yang, W., O’Neil, C., Wallace, M., Boon, A., … & Babcock, H. M. (2017). Defining Aerosol Generating Procedures and Pathogen Transmission Risks in Healthcare Settings. Open Forum Infectious Diseases, 4(1), 34-35. https://doi.org/10.1093/ofid/ofx162.085

[7] O'Dowd, K., Nair, K. M., Forouzandeh, P., Mathew, S., Grant, J., Moran, R., … & Pillai, S. C. (2020). Face Masks and Respirators in the Fight against the COVID-19 Pandemic: A Review of Current Materials, Advances and Future Perspectives. Materials (Basel, Switzerland), 13(15), 3363. https://doi.org/10.3390/ma13153363

[8] Shcherbenkov, I. M. (2013). Бактериофаги. Что мы знаем о них? [Bacteriophages. What do we know about them?]. Медицинский совет – Medical advice, 2-3, 56-63.

[9] Whiley, H., Keerthirathne, T. P., Nisar, M. A., White, M., & Ross, K. E. (2020). Viral Filtration Efficiency of Fabric Masks Compared with Surgical and N95 Masks. Pathogens (Basel, Switzerland), 9(9), 762. https://doi.org/10.3390/pathogens9090762

[10] Zagorodniuk, K., Zagorodniuk, Yu., Boyko, I., Wojciechowski, V., Korobochka, O., Kudryavtsev, S., & Novikov, M. (2021). Медичні маски із знезаражуючими властивостями [Medical masks with disinfectant properties]. Грааль науки – The Grail of Science, 8, 385-391. https://doi.org/10.36074/grail-of-science.24.09.2021.72
How to Cite
Klishch, I. M., Kovalchuk, A. O., Medvid, I. I., Pavlyshyn, A. V., & Klymnyuk, S. I. (2021). Evaluation of antibacterial and antiviral barrier function of the conceptual model of a pneumatic helmet. Reports of Vinnytsia National Medical University, 25(3), 466-468. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2021-25(3)-21