Microbiological grounds for antimicrobial treatment of experimental pseudomonal keratitis
Annotation. In the structure of the corneal microbial diseases bacterial keratitis take second rate position after viral keratitis. They are characterized by a severe course and often leads to complications that require surgical treatment. P. aeruginosa is the main causative agent of keratitis associated with the contact lenses wearing. The aim of our work was to check up the combined action of fluoroquinolones, tobramycin and antiseptic decamethoxine on clinical strains of P. aeruginosa in vitro with microbiological methods. The studies were performed on clinical strains of P. aeruginosa, which were sensitive as well as resistant to the studied antimicrobial compounds. Statistical analysis of the results was performed using standard software packages “STATISTICA +” and “Microsoft Excel 2010”. Calculated the arithmetic mean (Xcr), standard deviation (σ). The results of our investigation demonstrated 2–4 times antimicrobial susceptibility increasing for quinolone and tobramycin resistant clinical strains of Pseudomonas aeruginosa in the presence of subinhibitory concentrations of decamethoxine. Oftaquix, floximed and tobrex eye drops contain enough amount of antibiotics that is capable to affect both sensitive and resistant strains of P. aeruginosa in vitro. The revealed synergetic antipseudomonal action gives us grounds for further research of the combination etiotropic therapy effect on the course of the experimental keratitis caused by P. aeruginosa.
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