Investigation of the sensitivity of pathogens of purulent-inflammatory processes of the mediastinum to modern antiseptics


Keywords: antiseptic drugs, antimicrobial activity, purulent-inflammatory complications.

Abstract

Annotation. A well-chosen tactic for the prevention and treatment of infectious complications in patients with purulent-inflammatory processes of the mediastinum is important in reducing the colonization of the biotope by pathogens, contributes to the reduction of inflammation and accelerates recovery. The aim of our work was to conduct a comparative study of the antimicrobial efficacy of antiseptic drugs (decasan, myramistinum, chlorhexidine, povidone iodine and polyhexamethylene guanidine) on 278 clinical strains of microorganisms (S. aureus, E. coli, K. pneumoniae, Enterobacter spp.), which cause purulent-inflammatory complications of the mediastinum in patients. For the comparative characterization of the antimicrobial action, the method of double dilutions was used; calculated antiseptic activity index (AAI); a quantitative suspension test was performed to evaluate the decontamination effect of antiseptics. Standard methods of descriptive statistics and special and office programs “STATISTICA 6.0”, “Microsoft Excel 2010” were used. Staphylococci showed the highest level of sensitivity to most of the antiseptics under study. The mean MBSC values of S. aureus clinical strains for decasan, myramistinum, chlorhexidine was respectively 1.75±1.27 µg/ml, 3.25±2.27 µg/ml, and 5.53±3.74 µg/ml. The AAI of myramistinum and chlorhexidine before the Pseudomonas was 4.5, which is the threshold for clinical activity. The clinical activity of a solution of povidone iodine against infections caused by P. aeruginosa is doubtful, based on the activity level of 2.6. The studied antiseptics provided a full decontamination effect against most strains at 5 min exposure, the decontamination effect lasted for 15 min, followed by 30 min. There were found single microorganisms, the number of which gradually increased and after 1 hour reached 1.9×104 and 1.5×102 CFU/ml, respectively. The high sensitivity of gram-positive and gram-negative pathogens to decasan and polyhexamethylene guanidine hydrochloride substantiates their high antimicrobial effectiveness and opens wide prospects for their use in the prevention and treatment of infectious complications in modern conditions.

References

1. Kovalchuk, V. P. (2006). Kharakterystyka arsenalu antyseptychnykh zasobiv, zareiestrovanykh v Ukraini [Characteristics of the arsenal of antiseptic agents registered in Ukraine]. Biomedical and Biosocial Anthropology, 6, 71–76.

2. Kovalchuk, V. P., Isniuk, A. S., Vovk, I. M., Kishchuk, V. V., & Kovalenko, I. M. (2018). Porivnialna kharakterystyka dii pryrodnykh ta syntetychnykh antyseptychnykh preparativ na mikroorhanizmy, yaki zumovliuiut bakterialni infektsii verkhnikh dykhalnykh shliakhiv [Сomparative characteristics of the effect made by natural and synthetic antiseptics on microorganisms causing bacterial nasopharyngeal infections]. Visnyk Vinnytskoho natsionalnoho medychnoho universytet – Reports of Vinnytsia National Medical University, 22 (2), 257–261.

3. Nahaichuk, V. I., Nazarchuk, O. A., Palii, I. H., Burkot, V. M., & Honchar, O. O. (2014). Do kharakterystyky suchasnykh infektsiinykh uskladnen u khvorykh z opikamy [To characteristics of modern infectious complications in patients with burns]. Ukrainskyi medychnyi chasopys online – Ukrainian medical journal online, 5 (103). Vziato z https://www.umj.com.ua/article/79087/.

4. Nazarchuk, O. A. (2019). Doslidzhennia protymikrobnoi efektyvnosti suchasnykh antyseptychnykh zasobiv na osnovi dekametoksynu ta povidonu yodu [Research of antimicrobial efficacy of modern antiseptic agents based on decamethoxine and povidone-iodine]. Perioperative medicine, 2 (1), 4–10. DOI: 10.31636/prmd.v2i1.1.

5. Nazarchuk, O. A. (2016). Antyseptyky: suchasna stratehiia borotby zi zbudnykamy infektsiinykh uskladnen [Antiseptics: a modern strategy for combating infectious agents]. Klinichna khirurhiia – Clinical surgery, 9, 59–61. Vziato z http://nbuv.gov.ua/UJRN/KlKh_2016_9_18.

6. Nazarchuk, O. A., & Nahaichuk, V. I. (2015). Otsinka efektyvnosti zastosuvannia dekasanu, dekametoksynu ta yoho kompozytsii u patsiientiv z vazhkoiu termichnoiu travmoiu [Evaluation of the effectiveness of the use of decasan, decamethoxin and its composition in patients with severe thermal injury]. Annaly Mechnykivskoho instytutu – Annals of the Mechnikov Institute, 2, 184–190. Vziato z http://nbuv.gov.ua/UJRN/ami_2015_2_35.

7. Palii, V. H., Sukhliak, V. V., Palii, D. V., Honchar, O. O., Kryzhanovska, A. V., Bereza, B. M., … Zaderei, N. V. (2014). Protymikrobna diia atyseptychnykh preparativ, antybiotykiv na zbudnyky zapalnykh zakhvoriuvan [Antimicrobial action of antiseptic drugs, antibiotics on agents of inflammatory diseases]. Biomedical and Biosocial Anthropology, 22, 44–46. Vziato z http://nbuv.gov.ua/UJRN/bba_2014_22_12.

8. Rymsha, O. V., & Trofimenko, Yu. Iu. (2012). Chutlyvist mikroflory, izolovanoi v patsiientiv do antybiotykiv ta antyseaptykiv [Sensitivity of microbiota isolated in patients to antibiotics and antiseptics]. Biomedical and Biosocial Anthropology, 18, 38–41.

9. Faustova, M. O., Nazarchuk, O. A., & Ananieva, M. M. (2017). Etiolohichna struktura, biolohichni vlastyvosti dominuiuchykh zbudnykiv peryimplantatnoho mukozytu [The etiological structure, biological properties of causative agents of peri-implant mucositis]. Zaporozhskyi medytsynskyi zhurnal – Zaporozhye medical journal, 5, 652–657. Vziato z http://nbuv.gov.ua/UJRN/Zmzh_2017_19_5_25.

10. Abu-Omar, Y., Kocher, G. J., Bosco, P., Barbero, C., Waller, D., Gudbjartsson, T., … Cardillo, G. (2017). European Association for Cardio-Thoracic Surgery expert consensus statement on the prevention and management of mediastinitis, European Journal of Cardio-Thoracic Surgery, 51 (1), 10–29. https://doi.org/10.1093/ejcts/ezw326.

11. Imperatori, A., Nardecchia, E., Dominioni, L., Sambucci, D., Spampatti, S., Feliciotti, G., & Rotolo, N. (2017). Surgical site infections after lung resection: a prospective study of risk factors in 1,091 consecutive patients. Journal of thoracic disease, 9 (9), 3222–3231. https://doi.org/10.21037/jtd.2017.08.122.

12. Nazarchuk, O. A., Chereshniuk, I. L., & Nazarchuk, H. H. (2019). The research of antimicrobial efficacy of antiseptics decamethoxin, miramistin and their effect on nuclear DNA fragmentation and epithelial cell cycle. Wiadomosci Lekarskie, 72 (3), 374–380.
Published
2020-05-18
How to Cite
Shaprynskyi, V., Rymsha, O., Mitiuk, B., Vovk, I., Nazarchuk, S., Khodakivskyi, M., & Ivanova, M. (2020). Investigation of the sensitivity of pathogens of purulent-inflammatory processes of the mediastinum to modern antiseptics. Reports of Vinnytsia National Medical University, 24(1), 69-74. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2020-24(1)-13

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