Research of antimicrobial efficacy of a composition with prolonged antiseptic effect against planktonic and film forms of clinical strains of non-fermentative Gram-negative bacteria


Keywords: burn wound, biofilms, non-fermenting gram-negative bacteria, polymer antimicrobial composition.

Abstract

Annotation. The problem of treatment of burn wounds is closely related to the specificity of the spectrum of microorganisms that impair wound healing in this category of patients. A generally alarming trend in recent years is the significant increase in the etiological structure of wound infection, both in frequency and in severity and in the difficult choice of treatment tactics in the isolation of poly antimicrobial resistant strains of Pseudomonas aeruginosa, Acinetobacter baumannii. The aim of our study was to create a model of biofilm formed by bacteria on the wound surface and to investigate, in a comparative study, the sensitivity of planktonic and film forms of non-enzymatic gram-negative bacteria to a composition based on decamethoxin with prolonged antiseptic action. To determine the effect of the antiseptic composition on planktonic and film forms of bacteria there was used the method of two consecutive dilutions. The results of a comparative study of the activity of the antiseptic composition with decamethoxin planktonic and biofilm forms of strains of NFGB indicated that in the bacterial film bacteria were more resistant to drugs than in planktonic form. Film forms of A. baumannii and B. cepacia have a sensitivity to decamethoxin at a concentration of 13.6±1.95 µg/ml and 15.6±0 µg/ml, respectively. P. aeruginosa film forms have a higher degree of resistance to the test composition with decamethoxin (MBc 137.5±30.6 μg/ml). Disinfection of the planktonic form of NFGB is 10 times faster than the film form, the destruction of which is achieved with prolonged exposure of 120–150 minutes.

References

1. Balko, O. I., & Avdieieva, L. M. (2013). Etapy bioplivkoutvorennia Pseudomonas aeruginosa [Stages of biofilm Pseudomonas aeruginosa]. Ukrainian Food Journal – Ukrainian Food Journal, 2 (1), 23–26.

2. Zaslavskaya, N. V., Artemenko, N. K., Chizhevskaya, M. M., & Tec, V. V. (2000). Osobennosti vyzhivaemosti bakterij v mikrobnyh soobshestvah [Features of bacterial survival in microbial communities]. Klinich. mikrobiologiya i antimikrobnaya himioterapiya – Clinic. microbiology and antimicrobial chemotherapy, 2, 19–20.

3. Kondratiuk, V. M., Prokopchuk, Z. M., Burkot, V. M., & Vovk, I. M. (2018). Osoblyvosti formuvannia rezystentnosti do antybiotykiv u hramnehatyvnykh nefermentuiuchykh bakterii [Features of antibiotic resistance formation in gram-negative non-enzymatic bacteria]. Visnyk Vinnytskoho natsionalnoho medychnoho universytetu – Reports of Vinnytsia National Medical University, 22 (2), 253–257. DOI: 10.31393/reports-vnmedical-2018-22(2)-03.

4. Nahaichuk, V. I., Nazarchuk, O. A., & Palii, I. H. (2014). Do kharakterystyky suchasnykh infektsiinykh uskladnen u khvorykh z opikamy [Characteristics of modern infectious complications in patients with burns]. Ukrainskyi medychnyi chasopys – Ukrainian Medical Journal, 5 (103), 123–126. Vziato z http://nbuv.gov.ua/UJRN/UMCh_2014_5_31.

5. Nazarchuk, O. A., & Nahaichuk, V. I. (2015). Otsinka efektyvnosti zastosuvannia dekasanu, dekametoksynu ta yoho kompozytsii u patsiientiv z vazhkoiu termichnoiu travmoiu [Evaluation of the effectiveness of the use of decasan, decamethoxin and its composition in patients with severe thermal trauma]. Annaly Mechnykivskoho instytutu – Annals of the Mechnikov Institute, 2, 184–190. Vziato z http://nbuv.gov.ua/UJRN/ami_2015_2_35.

6. Palii, H. K., Nazarchuk, O. A., Nahaichuk, V. I., Vovk, I. M., & Nazarchuk, H. H. (2017). Obgruntuvannia dotsilnosti zastosuvannia dekametoksynu pry antybiotyko- ta fahorezystentnosti psevdomonadnoi khirurhichnoi infektsii [Substantiation of expediency of the use of decamethoxin for antibiotic and phage resistance of pseudomonas surgical infection]. Klinichna khirurhiia – Clinical Surgery, 9 (905), 64–67. Vziato z http://nbuv.gov.ua/UJRN/KlKh_2017_9_20.

7. Trofimenko, Yu. Yu., Makats, Ye. F., Stukan, O. K., & Burkot, V. M. (2018). Chutlyvist bioplivkovykh ta planktonnykh form nefermentuiuchykh bakterii do dii antyseptykiv [Sensitivity of biofilm and planktonic forms of non-enzymatic bacteria to the action of antiseptics]. Visnyk Vinnytskoho natsionalnoho medychnoho universytetu – Reports of Vinnytsia National Medical University, 22 (2), 293–297. DOI: 10.31393/reports-vnmedical-2018-22(2)-12.

8. Chebotar, I. V. (2012). Antibiotikorezistentnost bioplyonochnyh bakterij [Antibiotic resistance of biofilm bacteria]. Klinicheskaya mikrobiologiya i antimikrobnaya himioterapiya – Clinical Microbiology and Antimicrobial Chemotherapy, 14 (1), 51–58. Vzyato s https://cmac-journal.ru/publication/2012/1/cmac-2012-t14-n1-p051/cmac-2012-t14-n1-p051.pdf.

9. Burn incidence and treatment in the U.S.: 2007 Fact sheet. (2005). American Burn Association. (Accessed 14 Apr 2009). Retrieved from http://www.ameriburn.org/resources_factsheet.php.

10. Nahaichuk, V., & Nazarchuk, O. (2020). Correlation of Susceptibility to Antiseptics With Biofilm-forming Properties in Acinetobacter baumannii as a Pathogen of Surgical Infection. Malaysian Journal of Medicine and Health Sciences, 16 (1), 1–5.
Published
2020-05-18
How to Cite
Nazarchuk, O. A., Nahaichuk, V. I., Rymsha, O. V., Palii, V. H., Vovk, I. M., Bobyr, N. A., Prokopchuk, Z. M., & Stukan, O. K. (2020). Research of antimicrobial efficacy of a composition with prolonged antiseptic effect against planktonic and film forms of clinical strains of non-fermentative Gram-negative bacteria. Reports of Vinnytsia National Medical University, 24(1), 64-68. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2020-24(1)-12