Antimicrobial activity investigation of natural bromotyrosine derivatives
Annotation. The relevance of the search for new natural antimicrobials is dictated by global spread of antimicrobial resistance. Studies of marine sponges of the class Demospongiae, including more than 7300 species, have become promising from the pharmacological point of view. Sponges produce a wide range of biologically active substances of different classes such as steroids, isoprene, alkaloids, terpenoids, etc., which can inhibit the growth of pathogenic microorganisms or kill them. The trial of brominated isoxazoline alkaloid derivates named bromotyrosines has been enough promising. The aim of our work was to determine the antimicrobial activity of aeroplysinin-1 against clinical antibiotic-resistant strains of gram-positive bacteria and fungi of the genus Candida. The aeroplysinin-1 substance was isolated by methanol extraction from desiccated specimens of sponge Aplysina aerophoba followed with its separation by column chromatography in the Extreme Biomimetics Laboratory of the Electronics and Sensor Materials Institute, Freiberg Mining Academy, UT (Germany). Well method was used to determine antimicrobial action of the tested substance against clinical strains of bacteria and fungi (S. aureus, C. acnes, C. albicans). Aeroplysinin-1 was found to demonstrate high antibacterial activity against clinical strains of the genera Cutibacterium, Staphylococcus. The inhibiting growth zones around the wells filled with 0.1% solution of aeroplysinin-1 in dimethyl sulfoxide had diameters of 35.4±3.2 mm, 32.1±2.8 mm and 26.5±2.5 mm for cutibacterium, enterococci, and staphylococci, respectively. It was revealed aeroplysinin-1 had no activity against fungi of the genus Candida. So, after a comprehensive study of pharmacological properties of this natural bromotyrosine compound, which is a secondary metabolite of marine sponges Aplysina aerophoba, it can be added to the list of alternative compounds to struggle multiresistent to modern antibiotics pathogens causing human diseases.
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