Study of antimicrobial activity of Ruta graveolens L. garden root extracts against clinical strains of microorganisms
Annotation. The rapid growth rate of resistance of microorganisms to most antibacterial drugs forces scientists from different countries to search for new therapeutic agents that will have antimicrobial activity, as well as to which resistance of microorganisms will be slower. Promising in this situation are complexes of biologically active substances (BAS) of plant origin, which have a number of advantages over conventional antibiotics. Particularly noteworthy is the garden root (GR), which has a rich chemical composition and has a wide range of properties, including the presence of antimicrobial activity. The purpose of this study was to determine the activity spectrum of water-ethanol extracts of garden root relative to clinical strains of microorganisms with different sensitivity to antibiotics. The method of serial dilutions in the broth investigated the direct antimicrobial activity of 5 water-ethanol extracts and tincture of herb garden Ruta graveolens L. against 53 strains of microorganisms. Gene5 and Microsoft Office Excel 2011 were used for statistical processing of the results. Among the microorganisms were the most sensitive epidermal staphylococci, growth inhibition of 57.9% and 47.4% of the strains, which were observed at a dilution of 1:40 PC extracts of 50% and 70%, respectively. 90% of the GR extract showed its activity at a dilution of 1:20 relative to 57.9% of S. epidermidis strains. Moderate antifungal activity of MS extracts against yeast fungi of the genus Candida was also established. It is most expressed in 70% of the extract of MS, which at a dilution of 1:40 inhibited the growth of 66.7% of the strains. The active components of the garden root extracts being investigated, which showed a moderate antimicrobial and antifungal action, can be used to create new therapeutic agents for their treatment of skin infections.
2. Pavliuk, N. V., Kutsyk, R. V., & Yurchyshyn, O. I. (2019). Aktualni problemy mikrobiolohii, virusolohii ta imunolohii, Materialy naukovoi konferentsii [Actual problems of microbiology, virology and immunology, conference materials]. Vinnytsia: Nova knyha.
3. França Orlanda, J. F.б & Nascimento, A. R. (2015). Chemical composition and antibacterial activity of Ruta graveolens L. (Rutaceae) volatile oils, from São Luís, Maranhão, Brazil. South African Journal of Botany, 99, 103–106. DOI: 10.1016/j.sajb.2015.03.198.
4. Freiesleben, S. H., & Jäger, A. K. (2014). Correlation between Plant Secondary Metabolites and Their Antifungal Mechanisms – A Review. Medicinal & Aromatic Plants, 3, 154. DOI: 10.4172/2167-0412.1000154.
5. Haddouchi, F., Chaouche, T. M., Zaouali, Y., Ksouri, R., Attou, A., & Benmansour, A. (2013). Chemical composition and antimicrobial activity of the essential oils from four Ruta species growing in Algeria. Food Chemistry, 141, 253–258. DOI: 10.1016/j.foodchem.2013.03.007.
6. Koehn, F., & Carter, G. (2005). The Evolving Role of Natural Products in Drug Discovery. Nature Reviews Drug Discovery, 4, 206–220. DOI: 10.1038/nrd1657.
7. Subramani, R., Narayanasamy, M., & Feussner, K. D. (2017). Plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. 3 Biotech, 7 (3), 172. DOI: 10.1007/s13205-017-0848-9.
8. Tobyn, G., Denham, A., & Whitelegg, M. (2009). Ruta graveolens, rue. The Western Herbal Tradition E-Book: 2000 years of medicinal plant knowledge (pp. 283-295). Elsevier Ltd. DOI: 10.1016/8978-0-443-10344-5.00032-X.
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