The species composition of microflora of health care associated infections in the intensive care unit


Keywords: microflora, heath care associated infections, intensive care unit.

Abstract

Annotation. The aim of the study was to identify the predominant microorganisms that caused health care associated infections in the intensive care unit (for surgical patients) National Military Medical Center “Main Military Clinical Hospital” in 2018. Identification of microorganisms was carried out in the laboratory department (microbiological), clinic of laboratory diagnostics the same hospital, which has national accreditation for work with microorganisms of the III – IV class of pathogenicity. The results of 631 bacteriological examinations were analyzed, namely 240 samples of blood from 88 patients, 269 urine samples from 77 patients, 97 samples of endotracheal content/sputum, obtained from 16 patients and another 25 samples from other anatomical sites (the bile ductus drains, pleural fluids) from 8 patients. Statistical processing was performed using Microsoft Excel 2016. The significance of the difference was checked by the method of χ2. The number of samples without growth was 206 (33 %). There were founded that the leading microorganism was K. pneumoniae 105 (25 %) isolates, followed by P. aeruginosa with 80 isolates (19 %), P. mirabilis 19 (4 %), E. coli 18 (4 %). The fraction of gram-positive bacteria was 31 % (118 isolates). There is a prominent difference among predominant microorganisms that caused health care associated infections corresponding to the anatomic site infection (p˂0.05). In blood cultures the K. pneumoniae (26 %) were leading microorganism, while P. aeruginosa (24 %) was found predominant in the respiratory tract, and Enterococcus spp (23 %) were the main isolates from the urine. Any isolates of A. baumannii were cultured. Formalizing the process of bacteriological observation, careful selection of patients for research and collection of epidemiological information featuring health care associated infections in the intensive care unit will systematize the process of infection control and allow to apply a quality control system.

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Published
2020-05-18
How to Cite
Ganiuk, V., Khil, V., Kondratiuk, V., Francishco, A., & Polishchuk, L. (2020). The species composition of microflora of health care associated infections in the intensive care unit. Reports of Vinnytsia National Medical University, 24(1), 20-24. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2020-24(1)-04