Antibiotic resistant and phagosensitivity of regional strains-causative agents of pyo-inflammatory diseases (S. aureus and P. aeruginosa).


Keywords: antibioticresistance, phagosensitivity, bacteriophage drugs, S. aureus, P. aeruginosa.

Abstract

Annotation. The actual issue is studying of sensitivity to antibiotics of regional strains-causative agents when there is increasing circulation of poly-resistant microorganisms. They were isolated from different biotopes. Their sensitivity to various commercial bacteriophage preparations was carried out in parallel. The purpose of the study was to determine the antibiotics of choice for starting therapy of purulent-inflammatory diseases and the prospects of using bacteriophage preparations relative to the most etiologically significant regional pathogen strains. Common methods of investigation were used: disco-diffusion to determine antibiotics sensitivity and drop method to study phagosensitivity. The results were processed by the method of variational statistics using M. Excel 2000 Biostat with Student's t test. It has been shown that antibiotics of choice for starting therapy of purulent-inflammatory diseases, in the absence of bacteriological examination of patients and determination of antibiotic sensitivity of pathogens, it is advisable to consider fluoroquinolones (sensitivity of S. aureus and P. aeruginosa to ciprofloxacin, lomefloxacin, norfloxacin about 90%), amikacin and gentamycin (78,3–39,7%), imipenem (57,4%). The conducted studies confirm the prospect of using existing bacteriophage drugs, including “Pyofag” and “Intestyfag”, LLC “Farmaxgroup” (Ukraine). The sensitivity of regional strains was 50,0–60,0%. It remains promising to study the effectiveness of the complex using of antibiotics and bacteriophages and to improve the action of commercial preparation of bacteriophages.

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Published
2020-05-18
How to Cite
DеrкаchS., Gorodnicka, N., Kutsaj, N., Gabisheva, L., & КоtsarО. (2020). Antibiotic resistant and phagosensitivity of regional strains-causative agents of pyo-inflammatory diseases (S. aureus and P. aeruginosa). Reports of Vinnytsia National Medical University, 24(1), 6-11. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2020-24(1)-01