The prognostic value of immunocellular infiltration of non-invasive urothelial bladder cancer
The aggressiveness of urothelial bladder cancers can be caused by reactions of stroma. Immunocompetent cells, which involved in antitumor response, are also able to stimulate tumor growth. Aim of the study: to study the features of immuncellular infiltration of non-invasive urothelial bladder cancers (NIUBC) to identify criteria for recurrence and progression prediction. Materials and methods: we formed three groups of studies, each of them contained 14 cases: NIUBC without recurrence — Group I, primary NIUBC with recurrence, but without progression — Group II, and primary NIUBC with recurrence and with progression — Group III. Immunohistochemistry was performed using primary monoclonal antibodies from DAKO to CD3, CD4, CD8, CD20 and CD68 cells. Statistical processing of research results was performed using the “Statistica 6.0” package. The relationship between parameters was studied by non-parametric Pearson χ-square test and Spearman test. A value of p<0.05 was considered statistically significant. Results: NIUBC I group was associated with weak stromal infiltration of immunocompetent cells: CD3 + lymphocytes (p<0.05), CD8 + T-killers (p<0.005) and CD68 + macrophages (p<0.005). In cancers of group III, immune cell infiltration was more observed than in cancers of groups I and II (p<0.001 and p<0.03) and was characterized by infiltration of CD3 + (p<0.01) and CD8 + cells (p<0.03). Thus, differentiation and recurrence of NIUBC progression depends on infiltration with immunocompetent cells, which can serve as a criterion for the disease prognosis. The prospect of further research – We are going to in-depth our research with studying importance of microenvironment of NIUBC, namely, immune cellular reactions, neoangiogenesis, connective tissue component at molecular-biological level. It will allow us to predict more accurate determination of NIUBC prognosis and choice of adequate treatment tactics.
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