Dynamics of nuclear-cytoplasmic relations in cardiomyocytes in the rat`s heart in the early postnatal period in normal conditions and experiment


Keywords: morphology, cardiomyocytes, heart, prenatal exposure, dexamethasone.

Abstract

Dexamethasone is administered to pregnant women with high risk of preterm delivery, but his impact during intrauterine development can program progression of undifferentiated connective tissue dysplasia and a tendency to cardiovascular diseases in adulthood. The aim of the study was to determine the changes of the nuclear-cytoplasmic relations (NCR) of cardiomyocytes (CMC) in the myocardium in the postnatal period after intrauterine administration of dexamethasone. 144 hearts of laboratory rats were studied from 1 to 45 days of life, which were divided into 3 groups: first — intact group, animals of second group were injected by 0.05 ml dexamethasone solution on the 18th day of intrauterine development, and III — control group. Tissue sections were stained with hematoxylin and eosin. Statistical evaluation was carried out with Mann-Whitney U-test. p<0,05 was accepted as statistically significant. It has been found that, the NCR increases 1.56-fold from birth till 14-th day and decreases 2.55-fold till 45-th day in the myocardium of the left ventricle. There changes correspond for the transition from a proliferative to a hypertrophic growth, which occurs on 14-th day after birth in the heart of rodents. In animals of the experimental group, the NCR was significantly increased at 3, 5, 21, 30, and 45, but decreased on the 14-th day (p<0,01) in comparison with control group. The maximum value of the index was observed on the 5-th day, which indicates on early terminal differentiation of CMC in experimental animals. Thus, intrauterine administration of dexamethasone leads to early maturation of CMC, which reduces their number and may increase the risk of developing cardiovascular diseases in adulthood. In the future we plan to study the proliferative activity of CMC of rat`s heart in the postnatal period in normal conditions and after intrauterine administration of dexamethasone.

Author Biography

A.V. Cherniavskyi

Cherniavskyi Artur Valerievich, teaching assistant of department of Human anatomy, operative surgery and topographical anatomy ZSMU. https://orcid.org/0000-0002-3902-8081  +380662877794

References

1. Galaktionova, M. Yu., & Mironova, O. I. (2014). Osobennosti centralnoj gemodinamiki detej s sindromom displazii soedinitelnoj tkani serdca [Features of the central hemodynamics of children with dysplasia of the connective tissue of the heart]. Mat i ditya v Kuzbasse – Mother and child in Kuzbass, 1, 41–46.

2. Hnatiuk, M. S., Slabyi, O. B., & Tatarchuk, L. V. (2016). Yaderno-tsytoplazmatychni vidnoshennia u kardiomiotsytakh ta endoteliotsytakh shlunochkiv leheneveho sertsia [Nuclear-cytoplasmic relations in cardiomyocytes and endothelial cells of the ventricles of the pulmonary heart]. Klinichna anatomiia ta operatyvna khirurhiia – Clinical Anatomy and Operative Surgery, 15 (1), 67–70.

3. Zagorujko, G. E., & Zagorujko, Yu. V (2017). Vozrastnye izmeneniya razmerov i chisla kardiomiocitov, ih yader v processe prenatalnogo i rannego postnatalnogo razvitiya serdca krys. Visnyk problem biolohii i medytsyny – Bulletin of Biology and Medicine, 3 (4), 304–311.

4. Konovalenko, S. O. (2011). Vikovi osoblyvosti yaderno-tsytoplazmatychnykh spivvidnoshen u kardiomiotsytakh shlunochkiv sertsia doslidnykh tvaryn [Age-specific features of nuclear-cytoplasmic ratios in cardiomyocytes of the heart ventricles of experimental animals]. Shpytalna khirurhiia – Hospital Surgery, 4, 40–42.

5. Kuandykov, E. U., Dzhumasheva, R. T., Almuhambetova, S. K., & Zhumagul, M. Zh. (2018). Beremennost i lekarstva. Vestnik Kazahskogo Nacionalnogo medicinskogo universiteta – Bulletin of the Kazakh National Medical University, 1, 462–469.

6. Reznikov, A. G., & Nosenko, N. D. (2014). Eksperimentalnoe izuchenie otdalennyh posledstvij perinatalnogo primeneniya lekarstvennyh sredstv dlya zdorovya potomkov [An experimental study of the long-term effects of perinatal drug use on the health of descendants]. Neonatolohiia, khirurhiia ta perynatalna medytsyna – Neonatology, Surgery and Perinatal Medicine, 1 (11), 70–78.

7. Gay, M. S., Li, Y., Xiong, F., Lin, T., & Zhang, L. (2015). Dexamethasone Treatment of Newborn Rats Decreases Cardiomyocyte Endowment in the Developing Heart through Epigenetic Modifications. PLoS ONE 10 (4), e0125033. doi.org/10.1371/journal.pone.0125033.

8. Annelene Govindsamy, Strinivasen Naidoo, and Marlon E. Cerf. (2018). Cardiac Development and Transcription Factors: Insulin Signalling, Insulin Resistance, and Intrauterine Nutritional Programming of Cardiovascular Disease. Journal of Nutrition and Metabolism, 2018, 12. Article ID 8547976. doi.org/10.1155/2018/8547976.

9. Peng, J., Zhou, Y., Zhang, Z., Wang, Z., Gao, L., Zhang, X., … Gao, L. (2018). The detrimental effects of glucocorticoids exposure during pregnancy on offspring's cardiac functions mediated by hypermethylation of bone morphogenetic protein-4. Cell death & disease, 9 (8), 834. doi:10.1038/s41419-018-0841-1.

10. Ponnusamy, M., Li, P. F., & Wang, K. (2017) Understanding cardiomyocyte proliferation: an insight into cell cycle activity. Cellular and Molecular Life Sciences, 6 (74), 1019–1034. doi.org/10.1007/s00018-016-2375-y.

11. Sakurai, K., Osada, Y., Takeba, Y., Mizuno, M., Tsuzuki, Y., Ohta, Y., … Matsumoto, N. (2019). Exposure of immature rat heart to antenatal glucocorticoid results in cardiac proliferation. Pediatrics International, 61, 31–42. doi:10.1111/ped.13725.

12. World health statistics 2018: monitoring health for the SDGs, sustainable development goals. (2018). Geneva: World Health Organization. Retrieved from https://apps.who.int/iris/rest/bitstreams/1137482/retrieve.
Published
2019-03-01
How to Cite
Cherniavskyi, A. (2019). Dynamics of nuclear-cytoplasmic relations in cardiomyocytes in the rat`s heart in the early postnatal period in normal conditions and experiment. Reports of Vinnytsia National Medical University, 23(1), 89-93. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2019-23(1)-14