The study of the protective properties of vitamin E on bone marrow of rats in condition of the increased intake of heavy metals salts


Keywords: bone marrow, heavy metals, microelementosis, erythropoiesis, granulocytopoiesis, megakaryocyte.

Abstract

Homeostasis imbalance leads to the changes in qualitative and quantitative characteristics of all hematopoietic sprouts. It encourages to search for the effective protective means in the conditions of the polluted environment. Our research is focused on the study the protective properties of vitamin E on the bone marrow of the rats in conditions of the increased intake of the heavy metals salts. During the research the bone marrow of the 36 laboratory male rats was studied. The sections of the decalcified femoral bones were stained using hematoxylin and eosin method, Van Gieson’s technique and PAS-reaction, as well as immunohistochemical study to CD3, CD79α, S100 and MРО was used. Elemental composition of hematopoietic tissue was determined through atomic spectrophotometry. The accumulation of the heavy metals led to microelementosis (changes in qualitative and quantitative characteristics of all hematopoietic sprouts and surrounding tissues). The extension of the influence of the exogenous pollutants led to the suppression of erythro-and leucopoiesis, synchronous increase in the number of megakaryocytes. These indicators reached the significant deviations on the 90 day of the observation. The use of the vitamin E as a corrector reduces the negative effect of the pollutants that is demonstrated by the reduced accumulation of the heavy metals in the bone marrow, prevention of the significant deviations in the quantitative and qualitative composition of hematopoietic tissue.

Author Biographies

Y. Lyndina

Lyndina Yu.M. Assistant professor of the Morphology department at SSU. ORCID ID: 0000-0002-2132-0965.

V. Sikora

Sikora VV, аssistant professor of the Pathology department at SSU.  ORCID ID: 0000-0002-4147-6879.

N. Hyriavenko

Hyriavenko NI, PhD, Assistant professor of the Pathology department at SSU.  ORCID ID: 0000-0002-9805-014X.

М. Lyndin

Lyndin MS, PhD, Assistant professor of the Pathology department at SSU. ORCID ID: 0000-0003-4385-3903.

A. Romaniuk

Romaniuk AM, doctor of medical sciences, professor, head of the Pathology department at SSU.  ORCID ID: 0000-0003-2560-1382  +380507276193  v.sikora@med.sumdu.edu.ua

References

1. Brigelius-Flohé, R., & Traber, M. G. (1999). Vitamin E: function and metabolism. FASEB J., 13 (10), 1145–55. Retrievid from https://www.ncbi.nlm.nih.gov/pubmed/10385606.

2. Cline, J. M., & Maronpot, R. R. (1985). Variations in the histologic distribution of rat bone marrow cells with respect to age and anatomic site. Toxicol. Pathol., 13 (4), 349–55.

3. Grazuleviciene, R., Nadisauskiene, R., Buinauskiene, J., & Grazulevicius, T. (2009). Effects of Elevated Levels of Manganese and Iron in Drinking Water on Birth Outcomes. Polish J. of Environ Stud., 18 (5), 819–25.

4. Hirschi, K. K. (2012). Hemogenic endothelium during development and beyond. Blood., 119 (21), 4823–27. doi: 10.1182/blood-2011-12-353466.

5. Kim, E-S., Noh, S. K., & Koo, S. I. (1998). Marginal zinc deficiency lowers the lymphatic absorption of alpha tocopherol in rats. J. Nutr., 128, 265–70.

6. Niki, E., & Traber, M. G. (2012). A history of vitamin E. Ann. Nutr. Metab., 61 (3), 207–12. doi: 10.1159/000343106.

7. Romaniuk, A., Lyndina, Yu., Sikora, V., Lyndin, M., Karpenko, L., & Gladchenko, O. (2016). Structural features of bone marrow. Interv. Med. Appl. Sci., 8 (3), 121–6. doi: 10.1556/1646.8.2016.3.3.

8. Romaniuk, A., Sikora, V., Lyndin, M., Smiyanov, V., Sikora, V., Lyndina, Yu., … Korobchanska, A. (2017). The features of morphological changes in the urinary bladder under combined effect of heavy metal salts. Interv. Med. Appl. Sci., 9 (2), 105–11. DOI: 10.1556/1646.9.2017.2.09.

9. Tavian, M., & Péault, B. (2005). Embryonic development of the human hematopoietic system. Int. J. Dev. Biol., 49 (2–3), 243–50. DOI:10.1387/ijdb.041957mt.

10. Trumbo, P. R. (2005). The level of evidence for permitting a qualified health claim: FDA's review of the evidence for selenium and cancer and vitamin E and heart disease. J. Nutr., 135, 354–56. DOI:10.1093/jn/135.2.354.
Published
2019-03-01
How to Cite
Lyndina, Y., Sikora, V., Hyriavenko, N., LyndinМ., & Romaniuk, A. (2019). The study of the protective properties of vitamin E on bone marrow of rats in condition of the increased intake of heavy metals salts. Reports of Vinnytsia National Medical University, 23(1), 59-63. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2019-23(1)-09