The role of CD-20 positive b-lymphocytes in secondary kidney damage


Keywords: secondary kidney damage, CD20 B-lymphocytes.

Abstract

In recent years, has increased interest in the study of the new aspects of the function of B cells in chronic kidney disease, namely, the release of proinflammatory cytokines and chemokines, the presentation of antigen, activation of T cells, the role in tissue fibrosis, neolymphangiogenesis (i.e., de novo formation of lymphatic vessels) and ectopic lymphogenesis, the formation of tertiary lymphatic organs in places of inflammation of tissues. Our goal is to study the specificity and intensity of the expression of the immunohistochemical marker CD20 in secondary glomerulonephritis, which will give a new insight into the prognosis and relevance of the pathogenetically determined approach to treating these patients with contemporary targeting therapy. The new data suggests reflections on the role of these intra-lymphoid clusters of rich B-lymphocytes in a local immune response with subsequent fibrotic impairment in chronic kidney diseases. We conducted a study of kidney biopsy from 2014 to 2017. All the tissues we studied were stained according to generally accepted methods of histological and histochemical research. Immunohistochemical staining included monoclonal murine antibodies to CD20 (clone L26), rabbit polyclonal antibodies to IgA, IgG, IgM (DAKO). All slides were dyed with Mayer's hematoxylin. The data obtained by us provide an opportunity to state that the detection of a large number of CD20 positive B-lymphocytes in infiltrates of different localization in patients with autoimmune kidney impairment (all cases with vasculitis, some cases with SFV, patients with Goodpasture syndrome) is effective in prescribing specific biological treatment in form of the drug rituximab, which is a monoclonal antibody to the surface antigen of B-lymphocytes CD20. Cases with other secondary kidney lesions require further study of the role of CD20 positive B-lymphocytes in the pathogenesis of kidney changes.

Author Biographies

O.O. Dyadyk

Dyadyk Olena Oleksandrivna - Professor, MD, Dr. Sci., Head of department, Shupyk National Medical Academy of Postgraduate Education, Pathologic and Topographic Anatomy department Kiyv, Dorogozhyzka str., 9; phone 0444838663, alena0566@gmail.com ORCHID 0000-0002-9912-4286

Y.I. Beketova

Beketova Yulia Igorivna - P.h.d., assistant of department, Shupyk National Medical Academy of Postgraduate Education, Pathologic and Topographic Anatomy department Kiyv, Dorogozhyzka str., 9; phone 0678000711, julia04.03@rambler.ru ORCHID 0000-0001-8635-1802

N.M. Surgai

Associate professor, P.h.d., Head of department, Donetsk National Medical University, Pathomorphology, Forensic medicine Histology department

References

1. Alexopoulos, E., Seron, D., Hartley, R. B., & Cameron, J. S. (1990). Lupus nephritis: correlation of interstitial cells with glomerular function. Kidney Int., 37 (1), 100–109. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/1967662.

2. Aloisi F., & Pujol-Borrell R. (2006). Lymphoid neogenesis in chronic inflammatory diseases. Nat. Rev. Immunol., 6, 205–217.

3. Angeli, V., Ginhoux, F., Llodra, J., Quemeneu, L., Frenette, P. S., Skobe, M., … Randolph, G. J. (2006). B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity, 24 (2), 203–215. DOI:10.1016/j.immuni.2006.01.003.

4. Bizzaro N., Tozzoli, R., & Shoenfeld, Y. (2007). Are we at stage to predict autoimmunerheumaic diseases? Arthritis Rheum, 56 (6), 1736–1744. DOI:10.1002/art.22708.

5. Boucher, A., Droz, D., Adafer, E., Noe, L-H. (1986). Characterization of mononuclear cell subsets in renal cellular interstitial infiltrates. Kidney Int., 29, 1043–1049. Retrieved from https://core.ac.uk/download/pdf/82280879.pdf.

6. Browning, J. L. (2006). B cell move to centre stage: novel opportunities for autoimmune disease treatment. Nature Rev., 5 (7), 564–576. DOI:10.1038/nrd2085.

7. Cohen, C. D., Calvaresi, N., Armelloni, S., Schmid, H., Henger, A., Ott, U., … Kretzler, M. (2005). CD20-positive infiltrates in human membranous glomerulonephritis. J Nephrol., 18 (3), 328–333. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/16013025.

8. D’Agati, V. D., Appel, G. B., Estes, D., Knowles, D. M., & Pirani, C. L. (1986). Monoclonal antibody identification of infiltrating mononuclear leukocytes in lupus nephritis. Kidney Int., 30 (4), 573–581. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/3537453.

9. Edwards, J. C., Cambridge, G. (2006). B-cell targeting in rheumatoid arthritis and other autoimmune diseases. Nat. Rev. Immunol., 6 (5), 394–403. DOI:10.1038/nri1838.

10. Förster, R., Mattis, A. E., Kremmer, E., Wolf, E., Brem, G., & Lipp, M. (1996). A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell, 87 (6), 1037–1047. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/8978608.

11. Hooke, D. H., Gee, D.C., & Atkins, R. C. (1987). Leukocyte analysis using monoclonal antibodies in human glomerulonephritis. Kidney Int., 31 (4), 964–972. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/3495689.

12. Husby, G., Tung, K. S., Williams, R. C. Jr. (1981). Characterization of renal tissue lymphocytes in patients with interstitial nephritis. Am. J. Med., 70 (1), 31–38. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/7006393.

13. Kerjaschki, D., Regele H.M., Moosberger I., Nagy-Bojarski K., Watschinger B., Soleiman A., … Raab I. (2004). Lymphatic neoangiogenesis in human kidney transplants is associated with immunologically active lymphocytic infiltrates. J. Am. Soc. Nephrol., 15 (3), 603–612. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/14978162.

14. Martin, F. & Chan, A. C. (2006). B cell immunobiology in disease: evolving concepts from the clinic. Annu. Rev. Immunol., 24, 467–496. DOI:10.1146/annurev.immunol.24.021605.090517.

15. Murphy, P. M., Baggiolini, M., Charo, I. F., Hebert, C. A., Horuk, R., Matsushima, K., … Power, C. A. (2000). International Union of Pharmacology. XXII. Nomenclature for chemokine receptors. Retrieved from Pharmacol. Rev., 52 (1), 145–176. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/10699158.

16. Müller, G., & Lipp, M. (2003). Concerted action of the chemokine and lymphotoxin system in secondary lymphoid-organ development. Curr. Opin. Immunol., 15 (2), 217–224. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/12633673.

17. Müller, G. A., Markovic-Lipkovski, J., Frank, J., Rodemann H. P. (1992). The role of interstitial cells in the progression of renal diseases. J. Am. Soc. Nephrol., 2 (10), 198–205. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/1600137.

18. Rosai, J. (2011). Rosai and Ackerman’s Surgical Pathology [7-th ed.]. Elsevier Inc., 2306 p.

19. Sarwal, M., Chua, M. S., Kambham, N., Hsieh, S. C., Satterwhite, T., Masek, M., & Salvatierra, O. Jr. (2003). Molecular heterogeneity in acute renal allograft rejection identified by DNA microarray profiling. N. Engl. J. Med., 349 (2), 125–138. DOI:10.1056/NEJMoa035588.

20. Segerer, S., Banas, B., Wornle, M., Schmid, H., Cohen, C. D., Kretzler, M., … Grone, H. J. (2004). CXCR3 is involved in tubulointerstitial injury in human glomerulonephritis. Am. J. Pathol., 164 (2), 635–649. DOI:10.1016/S0002-9440(10)63152-5.

21. Segerer S., Mack, M., Regele, H., Kerjaschki, D., Schlondorff, D. (1999). Expression of the C-C chemokine receptor 5 in human kidney diseases. Kidney Int., 56 (1), 52–64. DOI:10.1046/j.1523-1755.1999.00544.x.

22. Segerer S., & Nelson, P. J. (2005). Chemokines in renal diseases. Sci. World J., 5, 835–844. DOI:10.1100/tsw.2005.105.

23. Segerer, S., Nelson, P. J., & Schlondorff, D. (2000). Chemokines, chemokine receptors, and renal disease: from basic science to pathophysiologic and therapeutic studies. J. Am. Soc. Nephrol., 11 (1), 152–176. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/10616852.

24. Shi, K., Hayashida, K., Kaneko, M., Hashimoto, J., Tomita, T., Lipsky, P. E., … Ochi, T. (2001). Lymphoid chemokine B cell-attracting chemokine-1 (CXCL13) is expressed in germinal center of ectopic lymphoid follicles within the synovium of chronic arthritis patients. J. Immunol., 166 (1), 650–655. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/11123349.

25. Strutz, F., & Neilson, E. G. (2003). New insights into mechanisms of fibrosis in immune renal injury. Springer Semin Immunopathol., 24 (4), 459–476. DOI:10.1007/s00281-003-0123-5.

26. Takemura, S., Braun, A., Crowson, C., Kurtin, P. J., Cofield, R. H., O’Fallon, W. M., … Weyand, C. M. (2001). Lymphoid neogenesis in rheumatoid synovitis. J. Immunol., 167 (2), 1072–1080. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/11441118.
Published
2019-03-01
How to Cite
Dyadyk, O., Beketova, Y., & Surgai, N. (2019). The role of CD-20 positive b-lymphocytes in secondary kidney damage. Reports of Vinnytsia National Medical University, 23(1), 36-40. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2019-23(1)-05