The role of CD-20 positive b-lymphocytes in secondary kidney damage

Keywords: secondary kidney damage, CD20 B-lymphocytes.


In recent years, has increased interest in the study of the new aspects of the function of B cells in chronic kidney disease, namely, the release of proinflammatory cytokines and chemokines, the presentation of antigen, activation of T cells, the role in tissue fibrosis, neolymphangiogenesis (i.e., de novo formation of lymphatic vessels) and ectopic lymphogenesis, the formation of tertiary lymphatic organs in places of inflammation of tissues. Our goal is to study the specificity and intensity of the expression of the immunohistochemical marker CD20 in secondary glomerulonephritis, which will give a new insight into the prognosis and relevance of the pathogenetically determined approach to treating these patients with contemporary targeting therapy. The new data suggests reflections on the role of these intra-lymphoid clusters of rich B-lymphocytes in a local immune response with subsequent fibrotic impairment in chronic kidney diseases. We conducted a study of kidney biopsy from 2014 to 2017. All the tissues we studied were stained according to generally accepted methods of histological and histochemical research. Immunohistochemical staining included monoclonal murine antibodies to CD20 (clone L26), rabbit polyclonal antibodies to IgA, IgG, IgM (DAKO). All slides were dyed with Mayer's hematoxylin. The data obtained by us provide an opportunity to state that the detection of a large number of CD20 positive B-lymphocytes in infiltrates of different localization in patients with autoimmune kidney impairment (all cases with vasculitis, some cases with SFV, patients with Goodpasture syndrome) is effective in prescribing specific biological treatment in form of the drug rituximab, which is a monoclonal antibody to the surface antigen of B-lymphocytes CD20. Cases with other secondary kidney lesions require further study of the role of CD20 positive B-lymphocytes in the pathogenesis of kidney changes.

Author Biographies

O.O. Dyadyk

Dyadyk Olena Oleksandrivna - Professor, MD, Dr. Sci., Head of department, Shupyk National Medical Academy of Postgraduate Education, Pathologic and Topographic Anatomy department Kiyv, Dorogozhyzka str., 9; phone 0444838663, ORCHID 0000-0002-9912-4286

Y.I. Beketova

Beketova Yulia Igorivna - P.h.d., assistant of department, Shupyk National Medical Academy of Postgraduate Education, Pathologic and Topographic Anatomy department Kiyv, Dorogozhyzka str., 9; phone 0678000711, ORCHID 0000-0001-8635-1802

N.M. Surgai

Associate professor, P.h.d., Head of department, Donetsk National Medical University, Pathomorphology, Forensic medicine Histology department


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How to Cite
Dyadyk, O., Beketova, Y., & Surgai, N. (2019). The role of CD-20 positive b-lymphocytes in secondary kidney damage. Reports of Vinnytsia National Medical University, 23(1), 36-40.