Mycobacterium of tuberculosis with defective cell wall, determined in the brain of the biological model with spongional changes

Keywords: tuberculosis infection, mycobacterium, acid resistant sticks, brain isolates.


Mycobacterium tuberculosis is endowed with resistance to adverse factors and rapidly forms drug resistance. The aim is to study of the connection of tuberculosis infection and the development of brain damage with signs of spongymorphic changes. There were investigated canned 10% formalin fragments of the brain of 2 goats with signs of central nervous system damage by histological, microbiological methods. For microbiological examination, 3–5 years brain samples after were sowed on the MycСel DW nutrient medium with a growth stimulator. The molecular genetic study was performed using a polymerase chain reaction on a Molecular Imager GelDoc TM XR + (BioRad) device. The polypeptide profile was studied electrophoretically. In the goats, who died with symptoms of central nervous system damage, spongiform changes were detected in the brain. In the brain samples, DNA and mycobacterium tuberculosis with a defective cell wall have been detected, accumulation of mycobacterial antigens has been observed in the cells of the brain and in the intercellular space. Despite the fact that brain samples were in 10% formalin for 1 month, 3 years and 5 years, in all cases mycobacterium tuberculosis with a defective cell wall was isolated. Their viability was comparable to the infectiousness of prions. The isolation of mycobacterium tuberculosis with a defective cell wall from the brain did not differ in morphology and polypeptide composition from isolates from tuberculin, FLK-BLV, lymph nodes of cows, patients with tuberculosis. This indicates a high probability that mycobacterial infection, depending on the infectious dose, the characteristics of the strain and host genome, as well as the state of the immune system, can cause oncogenic action, cause active tuberculosis, brain damage, and the cardiovascular system.

Author Biographies

O.P. Lysenko

Lysenko Alexander Petrovych – doctor of Veterinary, professor, the head of the Department of Molecular Biology of Institute of the experimental Veterinary Science n. a. S. N. Wyshelesski (Belarus, Minsk) Belarus, 223003, Minsk Briketa 28, e-mail

V.V. Vlasenko

Vlasenko Volodymyr Vasylovych – Doctor of Biological Sciences, Professor, Professor of the Department of Tourism and Hotel and Restaurant Affairs of the Vinnytsia Institute of Trade and Economics of Kyiv National University of Trade and Economics of the Ministry of Education and Science of Ukraine;; https//orcid. оrg/0000-0003-3674-4370.

H.K. Palii

Palii Hordii Kindratovych – Doctor of medical science, Professor, Professor of the Department of Microbiology, Virology and Immunology of National Pirogov Memorial Medical University,;

I.H. Vlasenko

Vlasenko Iryna Heorhiivna – Doctor of medical science, Professor, the head of the Department of Department of Commodity Studies, Examination and Commercial Entrepreneurship Vinnytsia Institute of Trade and Economics of Kyiv National University of Trade and Economics of the Ministry of Education and Science of Ukraine;; https//orcid. оrg/0000-0003-3674-4370.

O.A. Nazarchuk

Nazarchuk Oleksandr Adamovych – Ph.D., Associate Professor, Department of Microbiology, National Pirogov Memorial Medical University, Vinnytsya, Ministry of Public Health of Ukraine,; ORCID:; тел. 0977293761.


1. Vlasenko, V. V. (1998). Tuberkulez v fokuse problem sovremennosti [Tuberculosis in the focus of modern problems]. Vinnica: Nauka.

2. Lysenko, A. P., Vlasenko, V. V. & Broxmeyer, L. (2014). Fenomen izmenchivosti mikobakterij tuberkuleza i ego ispol'zovanie dlja obnaruzhenija tuberkuleznoj infekcii [The phenomenon of variability of mycobacterium tuberculosis and its use for the detection of tuberculosis infection]. Tuberkulez − global'naja katastrofa chelovechestva, Materialy I Mezhdunarodnoj zaochnoj nauchno-prakticheskoj konferencii. – Tuberculosis is a global catastrophe of humanity, Materials of the I International Correspondence Scientific and Practical Conference. Rostov-na-Donu.

3. Lysenko, A. P., Vlasenko, V. V., Lemish, A. P., Novik, T. P., Mihalevich, E. A. & Vlasenko, I. G. (2014). Vyjavlenie mikobakterij tuberkuleza v tkanjah s pomoshh'ju differencirujushhej immunoperoksidaznoj okraski. [Detection of mycobacterium tuberculosis in tissues using differentiating immunoperoxidase staining]. Tuberkulez i bolezni legkih. – Tuberculosis and lung disease, (10), 55−58.

4. Lysenko, A. P., Vlasenko, V. V., Krasnikova, E. L. & Broxmeyer, L. (2018). Mikobakterii obnaruzheny v kul’tural’noj zhidkosti pochki jembriona ovcy, inficirovannyh virusom bych'ego lejkoza. Jepizootologija, immunobiologija, farmakologija, sanitarija [Mycobacteria are found in the culture fluid of a sheep embryo kidney infected with bovine leukemia virus]. Mezhdunarodnyj nauchno-prakticheskij zhurnal RUP “IJeV im. S.N.Vyshelesskogo” – Epizootology, immunobiology, pharmacology, sanitation. International Scientific and Practical Journal RUE “IEVS n. a. S. N. Wyshelesski”, 1, 7–18.

5. Alavi, H. A. & Moscovic, E. A. (1996). Immunolocalization of cell-wall-deficient forms of Mycobacteria tuberculosis complex in sarcoidosis in sinus histiocytosis of lymph nodes draining carcinoma. Histol Histopathol., 11 (3), 683–694. Retrieved from

6. Almenoff, P. L., Johnson, A., Lesser, M. & Mattman, L. H. (1996). Growth of acid fast L forms from the blood of patients with sarcoidosis. Thorax, 51, 530–533. Retrieved from

7. Anestad, G. & Hoel, T. (2001). Atherosclerosis and tuberculosis: are they both chronic infectious diseases. Scand J Infect. Dis., 33, 797. Retrieved from

8. Beran, V., Havelkova, M., Kaustova, L., Dvorska, J. & Pavlik, I. (2006). Cell wall deficient forms of mycobacteria: a review. Veterinarni Medicina, 51 (7), 365–389. Retrieved from

9. Broxmeyer, L. (2004). Is mad cow disease caused by a bacteria. Medical Hypothesis, 63, 731–739. DOI: 10.1016/j.mehy.2004.04.013.

10. Broxmeyer, L. (2005). Diabetes mellitus, tuberculosis and the mycobacteria: two millennia of enigma. Medical Hypothesis, 65 (3), 433–439. DOI:10.1016/j.mehy.2005.04.017.

11. Broxmeyer, L. (2016). Alzheimer`s Disease – How Its Bacterial Cause Was Found and Then Discarded. Create Space, USA. ISBN-10: 1491287357; ISBN-13: 978-1491287354.

12. Gerston, K. F., Blumberg, L., Tshabalala, V. A. & Murray, J. (2004). Viability of myco-bacteria in formalin-fixed lungs. Hum. pathol., 35, 571–575.

13. Guliang, H. & Tefu, L. (1999). Mycobacterium tuberculosis L-forms. Microbial Ecology in Health and Disease, 10, 129–133.

14. Ghosh, J., Larsson, P., Singh, B., Pettersson, B. M. F., Islam, N. M., Sakhar, S. N. … Kisebom, L. (2009). Sporulation in mycobacteria. Proceedings of the National Academy of Sciences of the United States of America, 106 (26), 10781–10786. doi: 10.1073/pnas.0904104106.

15. Huaman, M., Henson, D., Ticona, E., Sterling, T. R. & Garvy, B. A. (2015). Tuberculosis and cardiovascular disease: linking the epidemics. Tropical Disease, Travel Medicine and Vaccines, 1, 10. doi: 10.1186/s40794-015-0014-5.

16. Mattman, L. (1993). Cell Wall Deficient Forms: Stealth Pathogens. Boca Raton: CRC Press. ISBN 9780849387678.

17. Lysenko, A. P., Vlasenko, V. V., Broxmeyer, L., Lemis, A. P., Novik, T. P. & Pritychenko, A. N. (2014). The tuberculin skin test: how safe is safe? The tuberculins contain unknown forms capable of reverting to cell-wall-deficient mycobacteria. Clinical and Experimental Medical Sciences, 2 (2), 55–73.

18. Roels, S. & Walravens, C. (2003). Mycobacterium bovis meningitis in a cow with clinical sings of BSE. Vet. Rec., 152 (26), 6807–6808. Retrieved from

19. Schartz, Ph. (1972). Amyloid degeneration and tuberculosis in the aged. Gerontologia, 8 (5–6), 321–362.

20. Slavchev, G., Michailova, L. & Markova, N. (2013). Stress-induced L-forms of M. bovis: challenge to survivability. New Microbiologica, 36, 157–166. Retrieved from

21. Song, L. Y., Yan, W. S. & Zhao, T. (2002). Detection of Mycobacterium tuberculosis in lung cancer tissue by indirect in situ nested PCR. Di Yi Jun Yi Da Xue Xue Bao, 22, 992–993. Retrieved from

22. Tian, Y., Hao, T., Cao, B., Zhanget, W., Ma, Y., Lin, Q. & Li, X. (2015). Clinical End-Points Associated with Mycobacterium tuberculosis and Lung Cancer: Implications into Host-Pathogen Interaction and Coevolution. BioMed Research Intern., 1–9. doi: 10.1155/2015/827829.

23. Tomiyama, T. & Satoshi, A. (1994). Rifampicin prevents the aggregation and neurotoxity of amyloid B protein in vitro. Biochem Biophis Res Commun, 204 (1), 76–83. DOI:10.1006/bbrc.1994.2428.

24. World Health Organization (2010). Report Global Tuberculosis Control. World Health Organization Library Cataloguing-in-Publication, 218, 16. Retrieved from

25. TIAN Yan-sheng, CUI Xing-kun, ZHANG Wei, MA Yan, DONG Sheng-feng, ZHAO Wen-yan … LI Hong-guang (2013). Detection of Mycobacterium tuberculosis L-forms and MPB64 gene in breast cancer tissues. J. of Practical Medicine, 15, 2552–2555. Retrieved from
How to Cite
Lysenko, O., Vlasenko, V., Palii, H., Vlasenko, I., & Nazarchuk, O. (2019). Mycobacterium of tuberculosis with defective cell wall, determined in the brain of the biological model with spongional changes. Reports of Vinnytsia National Medical University, 23(1), 12-19.

Most read articles by the same author(s)