Gender and age peculiarities of the level of hyaluronic acid in the blood serum of patients with chronic hepatitis C virus infection at different degrees of hepatic fibrosis
Timely diagnostics of the early stages of fibrosis in chronic hepatitis C infection and the possibility to control it in patients with liver cirrhosis remains a topical issue in modern medicine. Hyaluronic acid (HA) takes a special place among direct hepatic fibrosis markers and is largely related to fibrogenesis, but is also involved in fibrinolysis, reflecting extra-cellular matrix remodeling. The aim of our research is to specify hepatic fibrosis diagnostics in patients with chronic hepatitis C infection through determination of gender and age peculiarities of the hyaluronic acid level in the blood serum. 66 patients with CHC of the 1st virus genotype have been examined. The diagnosis is confirmed by PCR results (RNA HCV +). The control group included 21 healthy individuals. The patients were also distributed by age and gender. All patients and control group individuals underwent ultra-sound fibroesophagography using FibroScan device. The results of fibroesophagography were evaluated by the METAVIR scale. The level of HA in the blood serum was determined both for patients with CHC and control group individuals with a set ‘HyaluronicAcid’ (Corgenix, Inc., the USA)). Statistical processing of the results was carried out in the statistical package “STATISTICA 5.5” (licensed number AXXR 910А374605FA). A method of correlation-regression analysis was used to develop a model of the dependence of the level of GA (on the duration (stage) of triple antiviral therapy). The validity of the difference between the comparable sample results was carried out according to the Student's criterion. Revealed that the level of HA in the blood serum of patients with CHC was higher than in the group of healthy individuals. The HA level in the blood serum of patients with CHC of the younger age group was lower than in people of the older age group. The analysis made by us showed that the HA level in the blood serum of patients with CHC increased in correspondence with the intensification of fibrotic changes in liver tissue in both age groups. So, a tendency was traced to the increase of HA in the blood serum of patients with CHC as compared to healthy individuals (2.61 times in the younger age group and 2.82 times — in the older age group), as well as, respectively, intensification of fibrotic changes in liver tissue in both age groups (2.03–4.26 times in the younger age group and 2.15–4.83 — in the older age group). No reliable difference between the levels of HA in the blood serum of patients with CHC depending on the gender has been traced.
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