Prediction of the dysbiosis development risk by indicators of the condition-pathogenic microflora index

Keywords: bacterial vaginosis, dysbiosis, conditionally pathogenic microflora.


Bacterial vaginosis is one of the most common types of infectious pathology of the reproductive organs of women mainly in reproductive age. The frequency of bacterial vaginosis over the past decade has doubled, ranging from 26% to 40–45%. Until now, the leading role in the emergence of bacterial vaginosis of obligate anaerobic bacteria has been proved, which is why it is regarded as a polymicrobial vaginal syndrome and is characterized not only by vaginal discharge, but also by damage to the cervix, the uterus, its appendages, childbirth. The etiological structure of pathogens of infectious processes in the last decade has changed significantly, which is due to the constant evolution of bacteria and the involvement of conventionally pathogenic microorganisms into pathological processes. The aim of the study is to identify the most informative indicators that objectively reflect the condition of the pathological process and develop a system for predicting the risk of occurrence and severity of dysbiosis behind these indicators. Studies were conducted in 298 women aged 16 to 64 years. 53 of whom were diagnosed with normocenosis, and 245 had dysbiosis. Statistical processing of data was carried out using variational and correlation analysis methods using the Application software package Statistica v.10 (StatSoft, Inc.). At the first stage of the analysis, the index of conditionally pathogenic microflora was considered as a resultant trait. To identify factors that are more associated with the risk of developing dysbiosis with IСPM, a selection of significant traits was performed using a genetic selection algorithm. At the second stage of the analysis, the prediction of the severity of dysbiosis with IСPM was considered. The nine factor attributes obtained with the help of mathematical analysis allowed us to predict the severity of vaginal dysbiosis with high accuracy and to calculate the IСPM indices. In addition, the phase of the development of the immune system reaction is shown in the development of vaginal dysbiosis — from the control state during normocoenosis to the development of immunoresistance in dysbiosis of grade 1 and expressed combined immunodeficiency in the presence of specific humoral response to bacterial antigens in dysbiosis of the 2nd degree.


1. Kafarskaya, L. I., Efimov, B. A., & Pokrovskaya, M. S. (2005). Mikroekologiya vlagalisha: Mikrobiocenoz v norme, pri patologicheskih sostoyaniyah i sposoby ego korrekcii. Lekciya [Microecology of the vagina: Microbiocenosis in normal, pathological conditions and the ways of correction. Lecture]. M.: Medicina – Moscow.

2. Kira, E. F. (2001). Bakterialnyj vaginoz [Bacterial vaginosis]. SPb: Neva-Lyuks – Saint Petersburg.

3. Kuzmin, V. I. (2003). Sovremennye aspekty terapii kandidoznogo vulvovaginita [Modern aspects of therapy of candidial vulvovaginitis]. Ginekologiya – Gynecology, 5 (3), 94–95.

4. Makarov, O. V., Bahareva, I. V., & Taranec, A. N. (2004). Sovremennye predstavleniya o vnutriutrobnoj infekcii [Modern views on the prenatal infection.]. Akusherstvo i ginekologiya – Оbstetrics and gynecology, 1, 10–13.

5. Molochkov, V. A., & Kirichenko, I. M. (2004). Polimeraznaya cepnaya reakciya i ee primenenie dlya diagnostiki v dermatovenerologii [Polymerase chain reaction and its applications for diagnostics in dermatovenerology]. Rossijskij zhurnal kozhnyh i venericheskih bolezne – Russian Journal of Skin and Venereal Diseases j, 1, 48–50.

6. Olina, A. A., & Loginova, N. P. (2007). Infekcionno-vospalitelnye zabolevaniya vlagalisha bakterialno-gribkovoj etiologii v eksperimente [Experimental Infectious-inflammatory diseases of the vagina with bacterial-fungal etiology]. Vestnik Rossijskoj voenno-medicinskoj akademii – Herald of the Russian Military Medical Academy, 1(17), 487–488.

7. Mendonca, K., Costa, C., Ricci, V., & Pozzi, G. (2015). Enzymatic assay to test diamines produced by vaginal bacteria. New Microbiology, 38 (2), 267–270. Retrieved from

8. Hickey, R. J., & Forney, L. J. (2014). Gardnerella vaginalis does not always cause bacterial vaginosis. J. Infect. Dis., 210(10), 1682–1683. doi: 10.1093/infdis/jiu303.
How to Cite
Gruzevskiy, A., Shevchuk, G., & Dubina, A. (2018). Prediction of the dysbiosis development risk by indicators of the condition-pathogenic microflora index. Reports of Vinnytsia National Medical University, 22(2), 333-337.