The research of the susceptibility to antimicrobial medicines of Acinetobacter baumannii as pathogens of infectious complications in patients with hard burns


Keywords: Acinetobacter baumannii, antibiotics, susceptibility, burns, resistance.

Abstract

Antibiotic-resistant strains of Acinetobacter baumannii has become yet recognized one of the most leading causative pathogens of infectious complications in patients with severe burns. This greatly complicates the treatment of such patients and requires in-depth study with a prognostic determination of the dynamics of antimicrobial efficacy of antibacterial agents. The aim — to study the susceptibility to antibiotics in clinical isolates of A. baumannii, pathogens of infectious complications in patients with hard burns. From patients (n=435) with burns of the 2ndb – 3rd degree, isolates of A.baumannii were received in early period after burn trauma before antibiotic therapy. Patients who participated in the study received standard surgical, complex general and local treatment in the required volume according to the protocols for the treatment of this disease. In total, 222 clinical strains of A.baumannii were isolated and identified during 2011–2016. The susceptibility of clinical strains A.baumannii to the following beta-lactam antibiotics: ampicillin-sulbactam, amoxicillin-clavulanate, piperacillin-tazobactam, cefoperazone-sulbactam, imipenem, meropenem, was determined by standard microbiological methods (qualitative disco-diffusion and quantitative double dilution methods). Using statistical methods, mathematical and analytical prognosis of the real sensitivity of A. baumannii strains to these antibiotics with the use of licensed computer programs “STATISTICA 7” was carried out; “Matlab 7.11”. The results of the study demonstrated a low susceptibility of clinical strains of A.baumannii to the studied beta-lactam antibiotics. The change in the antibiotic susceptibility profile of A.baumannii in 2011–2016 was established. Thus, the vast majority of strains were of low susceptibility to cefoperazone-sulbactam (55,6%), imipenem (57,1%) and meropenem (52,8%). In the dynamics, the prognostic decrease of the sensitivity in clinical isolates of Acinetobacteria to the antibiotics has been proved. The susceptibility of A.baumannii to amoxicillin-clavulanate was consistently low (less than 13,3%), and significantly decreased to cefoperazone–sulbactam (25,0%). The resistance of this pathogen to imipenem (up to 75,0%), meropenem (up to 84,3%) has been established to increase. At the same time, the gradual restoration of the susceptibility of A.baumannii to ampicillin–sulbactam (from 3,4% to 70,2%) was determined. The A.baumannii, pathogens of infectious complications in patients with burns, were characterized by a decrease in sensitivity to inhibitor-protected penicillins, carbapenems except ampicillin–sulbactam, that proved their low effectiveness against this pathogen.

References

1. Nazarchuk, O. A., Nagaychuk, V. І., Palіy, І. G., Burkot, V. M.& Gonchar, O. O. (2014). Do kharakteristiki suchasnikh іnfektsіynikh uskladnen' u khvorikh z opіkami [To characteristics of modern infectious complications in patients with burns]. Ukraїns'kiy medichniy chasopis, 5 (103), 123–126. [

2. Nekrasova, L. S., Svita, V. M., Glushkevich, T. G., Tomchuk, V. V., Zherebko, N. M., Yanovs'ka, V. V. … Pokas, O. V. (Ed.). (2007). Viznachennya chutlivostі mіkroorganіzmіv do antibakterіal'nikh preparatіv [The study of sensitivity of microorganisms to antibacterials: methodical recommendations]. Kyiv. MOZ Ukrainy. Retrieved from: http: // mozdocs.kiev.ua.

3. Palіy, G. K., Nazarchuk, O. A., Nazarchuk, G. G., Palіy, D. V., Kordon, Yu. V., Gonchar, O. O. … Zaderey, N. V. (2014). Doslіdzhennya chutlivostі zbudnikіv gnіyno-zapal'nikh zakhvoryuvan' do suchasnikh antimіkrobnikh preparatіv [The research of pathogens’ sensitivity to modern antimicrobial medicines at purulent-inflammatory diseases]. Zhurnal vushnikh, nosovikh і gorlovikh khvorob – Journal of the ear, nose and throat diseases, 1, 52–57.

4. Potemkin, V. G. (1999). Sistema inzhenernykh i nauchnykh raschetov МАТLАB [System of engineering and scientific calculations МАТLАB] 5.х. (Vol. 1-2). М. DIALOG-MIFI.

5. Yunkerov, V. I., Grigor'ev, S. G. & Rezvantsev, M. V. (2011). Matematiko-statisticheskaya obrabotka dannykh meditsinskikh issledovani [Mathematical and statistical processing of medical research data]. Sankt-Peterburg, SPb: VMeedA.

6. Leclercq, R., Cantón, R., Brown, D. F., Giske, C. G., Heisig, P., MacGowan A. P. … Kahlmeter, G. (2013). EUCAST expert rules in antimicrobial susceptibility testing. Clinical Microbiology Infection, 19 (2), 141–160. doi: 10.1111/j.1469-0691.2011.03703.x.

7. Nazarchuk, O. A. & Paliy, D. V. (2017). Substantiation of overcoming of antibiotic resistance in Acinetobacter baumannii clinical strains by usage of decamethoxinum. Annals of Mechnikovs Institute, 2, 28–33. Retrieved from http://nbuv.gov.ua/UJRN/ami_2017_2_7.
Published
2018-12-05
How to Cite
Nahaichuk, V., Nazarchuk, O., Osadchuk, N., Palyi, D., Nazarchuk, H., Köenig, E., Sorokoumova, L., & Honchar, O. (2018). The research of the susceptibility to antimicrobial medicines of Acinetobacter baumannii as pathogens of infectious complications in patients with hard burns. Reports of Vinnytsia National Medical University, 22(2), 306-310. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2018-22(2)-15

Most read articles by the same author(s)

1 2 > >>