Study of Alnus incana L. Moench fruit extract biologically active substances influence on resistance development in mls-resistant staphylococci skin isolates


Keywords: bacteria, resistance to antibiotics, macrolides, Alnus incana L.

Abstract

The emergence of microorganisms resistant strains is a natural biological response to the use of antimicrobial drugs that creates selective pressure, contributing to pathogens selection, survival and reproduction. The purpose of the investigation was to study the resistance development of staphylococci skin isolates to erythromycin and influence on it Alnus incana L. fruit extract subinhibitory concentrations. Development of resistance to erythromycin and influence on it Alnus incana L. fruit extract (extraction by 90% ethanol) subinhibitory concentrations were conducted with S epidermidis strains: sensitive and resistant to 14 and 15-membered macrolides. The study was carried out within 30 days by multiple consecutive passages of staphylococci test strains (concentration 1×107 CFU/ml) into test tubes containing broth and erythromycin ranging from 3 doubling dilutions above to doubling dilutions below the minimum inhibitory concentration. Statistical analysis of the results was carried out by one-and two-factor analysis of variance (ANOVA) and Microsoft Office Excel 2011. Rapid increase of resistance from 32 to 1024 μg/ml (F=34.2804; F> Fstand. max = 5.9874; p=0.0011) for S.epidermidis with a low level of resistance to 14 and 15-membered macrolides resistance to the erythromycine was observed. In the presence of Alnus incana L. fruit extract subinhibitory concentrations (¼ MIC), the initial MIC of erythromycin was decreased by 32 times to 1 μg/ml (F = 9.7497; F> Fstand. max = 5.9874; p = 0.0205). The sensitive strain after 30 passages did not develop resistance to erythromycin. Under the influence of erythromycin selective pressure, S.epidermidis strain with low initial level of MLS-resistance rapidly reaches a high-level resistance. Biologically active substances of the Alnus incana L. fruit extract significantly inhibit the resistance development in S. epidermidis to macrolides and eliminate it phenotypic features.

References

1. Kashpur, N. V. (2012). Protymikrobna aktyvnist i biolohichna diia substantsii roslynnoho pokhodzhennia roslyn rodiv Galium L. ta Artemisia L. [Antimicrobial activity and biological action of plant-derived substances of plants genera (Diss. of Ph.D., Medical Science)]. (Dys. kand. med. nauk). Instytut mikrobiolohii ta imunolohii imeni I.I. Mechnykova AMN Ukrainy, Kharkiv – Institute of Microbiology and Immunology named after I.I. Mechnikov Academy of Medical Sciences of Ukraine, Kharkiv.

2. Boos, M., Mayer, S., Fischer, A., Kohrer, K., Scheuring, S., Heisig, P. … Chmitz, F.-J. (2001). In vitro development of resistance to six quinolones in Streptococcus pneumonie, Streptococcus pyogenes, and Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 45 (1), 938–942. DOI:10.1128/AAC.45.3.938-942.2001.

3. Derakhshan, S., Sattari, M. & Bigdeli, M. (2010). Effect of cumin (Cuminum cyminum) seed essential oil on biofilm formation and plasmid integrity of Klebsiella pneumoniae. Pharmacogn. Mag., 6 (21), 57–61. doi: 10.4103/0973-1296.59967.

4. Hisatsune, J., Hirakawa, H., & Yamaguchi, T. (2013). Emergence of Staphylococcus aureus carrying multiple drug resistance genes on a plasmid encoding exfoliative toxin B. Antimicrob. Agents Chemother., 57 (12), 6131–6140. doi: 10.1128/AAC.01062-13.

5. Matsuoka, M., Endou, K., Kobayashi, H., Inoue, M. & Nakajima, Y. (1998). A plasmid that encodes three genes for resistance to macrolide antibiotics in Staphylococcus aureus. FEMS Microbiol. Lett., 167 (2), 221–227. https://doi.org/10.1111/j.1574-6968.1998.tb13232.x.

6. Murina, V., Kasari, M. & Hauryliuk, V. Antibiotic resistance ABCF proteins reset the peptidyl transferase centre of the ribosome to counter translational arrest. Nucleic Acids Research, 1(1), 1–11. doi: 10.1093/nar/gky050.

7. Yap, P. S., Lim, S. H. & Hu, C. P. (2013). Combination of essential oils and antibiotics reduce antibiotic resistance in plasmid-conferred multidrug resistant bacteria. Phytomedicine, 20(8), 710–713. doi: 10.1016/j.phymed.2013.02.013.

8. Schelz, Z., Molnar, Z., & Hohmann, J. (2006). Antimicrobial and antiplasmid activities of essential oils. Fitoterapia, 77 (4), 279–285. DOI:10.1016/j.fitote.2006.03.013.

9. Shimizu, T., Harada, K., & Kataoka, Y. (2013). Mutant prevention concentration of orbifloxacin: comparison between Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudointermedius of canine origin. Acta Veterinaria Scandinavica, 55 (37), 1–7. doi: 10.1186/1751-0147-55-37.
Published
2018-12-05
How to Cite
Kutsyk, R., & Yurchyshyn, O. (2018). Study of Alnus incana L. Moench fruit extract biologically active substances influence on resistance development in mls-resistant staphylococci skin isolates. Reports of Vinnytsia National Medical University, 22(2), 263-266. https://doi.org/https://doi.org/10.31393/reports-vnmedical-2018-22(2)-05