Influence of long-term using of soft contact lenses on indicators of oxidative-reducing potential of glutathione, peroxidation of lipids, and the state of cell membranes and cellular structures of the epithelium of the cornea
Contact lenses are increasingly used worldwide for correcting refraction disorders. However, contact lenses can destroy a biochemical composition of the tear. Purpose — to determine the effect of long-term use of silicone-hydrogel contact lenses on the parameters of the oxidation-reduction potential of glutathione, lipid peroxidation and the stability of cell membranes and subcellular structures of the cornea of the epithelium by identifying marker enzymes in a tear. We determined the activity of lactate dehydrogenase, glucose-6-phosphate dehydrogenase, acid phosphatase, succinatе dehydrogenase, glutathione (recovered and oxidized forms) and products of lipid peroxidation (malonic dialdehyde, diene conjugates) in tears of patients divided into two groups. Study group comprised 72 people — mild and moderate myopia patients continuously wearing soft contact lenses. Control group consisted of 33 people — mild and moderate myopia patients, spectacles wearers. We revealed a significant increase in the activity of lactate dehydrogenase and glucose-6-phosphate dehydrogenase by >33% and >26%, respectively, as well as a significant increase in the activity of acid phosphatase by >21% and of succinatе dehydrogenase by >18% in the tudy group patients comparing to controls. The study of the level of products of lipid peroxidation in the tear fluid of the main group showed a significant accumulation of them, as well as a violation of the glutathione balance in comparison with the control group. The dependence of biochemical changes in the composition of the tear on the duration of contact lens wear has been revealed. Therefore, with prolonged wearing of MKL, prooxidant-antioxidant equilibrium in the tissues of the eye surface, in particular in the cornea, is violated, activation of free radical processes and reduction of antioxidant reserves occur, which leads to destruction of cellular and subcellular membranes. We assume that the medical correction of these pathological changes will help prevent the development of severe complications of contact correction.
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