Morphological and morphometric analysis of tissue changes during implantation of polypropylene mesh implants modified with carbon nanotubes and antiseptics
Despite the introduction of modern materials for the plasticity of tissues in hernia, the results of treatment of this pathology are not entirely satisfied with the surgeons. A large number of complications and hernias recurrences, encourages the search for materials that would not cause tissue reactions to the foreign body and would have the proper antimicrobial properties. We have developed new mesh implants from modified polypropylene with carbon nanotubes and an antiseptic. The aim of the study is to conduct a comparative analysis of the tissue response to the implantation of mesh implants from polypropylene modified with carbon nanotubes and the antiseptic polyhexamethylene guanidine chloride. During the research, international norms and laws of Ukraine on bioethics adhered to. The study was performed on 70 laboratory rats in two series of experiments on 35 animals in each. In the first series, a polypropylene mesh was implanted, and in the second — a developed mesh. The animals were withdrawn from the experiment 3, 7, 14, 21, 30 and 90 days from the start of the experiment, and the material was taken for study. The selected abdominal wall tissues along with the mesh implants were fixed, poured into paraffin and slides were prepared, stained with hematoxylin-eosin and van Gieson, after what was examined under a microscope. The obtained digital data of the studies, in both series of experiments, were compared and subjected to statistical processing. The conducted studies made it possible to establish that caused inflammation by the implantation of the developed nanocomposite implants in the tissues disappear more rapidly than due to classical implants from polypropylene. At the same time, the formation of a connective tissue capsule that dissociated the implants from the tissues was completed before the 14th day of the experiment. At that time around the classical polypropylene implants during this observation period in the tissues minor inflammatory phenomena were observed, and the formation of the connective tissue capsule was completed until to 30 days of observation. So, high biocompatibility of the developed implants with tissues, what creates the prospect of their application in the clinic for the plastic of tissues was experimentally established.
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