Features of the state of immunity of immature rats with chronic rifampicin-isoniazid induced hepatitis, their correlation with the thymus and spleen cell cycle indexes


  • O. Guminska
  • N. Rikalo
Keywords: liver, chronic drug-induced hepatitis, transforming growth factor-β1, thymus cell cycle, cell cycle splenic T lymphocytes, immature rats, rifampicin, isoniazid,

Abstract

A significantly lower, compared with the intact group, relative number of CD3 + at 26,79% for the CD4 + 18,83% for CD8 + 50,66% + SD22 to 36,34% were foundеd in chronic drug-induced hepatitis. While using quercetin found that the relative number of T-lymphocytes compared to DILD, was significantly higher: CD3 + at 34,15% for CD4 + 18,4% CD8 + at 92,56%, CD22+ at 49,05%. In applying the "Antral" found a significant increase in the number of CD3 + at 24,34 % CD4 + 12 % CD8 + at 29.73 %, and significantly higher level of CD22 + at 70,75 %, compared with the same indicators in DILD. DNA cytometry thymus cells nuclei of immature rats with the DILD has established a significant increase percentage in thymus cell nuclei in G2M phase, 38 %, proliferation index more on 29,8 % and on 42 % more thymus cells nuclei with the fragmentation of nuclear DNA. Quercetin using significant increase in 26,6 % the percentage of nuclei thymus cells in S phase and leads to reduction in 56.4 % the percent of nuclei with DNA fragmentation signs in comparing with DILD. The use of "Antral" has led to increase in 31,5 % the percentage of thymus cell nuclei in the phase G2M, proliferation index on 16,2%. DNA cytometry analysis found a significant decrease in 4,6 % the percentage of nuclei in the spleen cells G0-G1 and increase in 13% the proliferation index and RN1 interval in 4 times. When using quercetin well established that the percentage of nuclei spleen cells with signs of DNA fragmentation at 42 % lower and when using the "Antral" 28 % lower than in untreated animals.

Published
2017-09-29
How to Cite
Guminska, O., & Rikalo, N. (2017). Features of the state of immunity of immature rats with chronic rifampicin-isoniazid induced hepatitis, their correlation with the thymus and spleen cell cycle indexes. Reports of Vinnytsia National Medical University, 19(1), 70-73. Retrieved from https://reports-vnmedical.com.ua/index.php/journal/article/view/168