Development and use of the modified Saburo medium for the isolation of Candida strains from biological material from patients

  • J.V. Sоbkоvа
  • A.A. Frantsishko
  • G.V. Filonenko
  • M.O. Rosada
  • A.I. Mihienkova
Keywords: candidiasis, fungi Candida species, isolate, Sabouraud medium.


In the detection of clinically important yeasts, the traditional use of Sabouraud medium for microbial isolation from the clinical specimens from the wards patients of multidisciplinary hospital often leads to false-negative results. There was a 10.8%-increase of yeast isolates if we used the complemented with yeast extract modified Sabouraud medium compared to classic Sabouraud medium. The use of modified Sabouraud medium could also significantly reduce time (1-2 days) required for the process of yeasts identification.


1. Beloborodova, N. V. & Vostrikova, T. Yu. (2009). Monitoring gribkovyh infekcij v ORiT. Klinicheskaya mikrobiologiya i antimikrobnaya himioterapiya, 1, 22–30. [in Russian].

2. Vybornova, I. V. (2009). Vozbuditeli kandidemii v Sankt-Peterburge. Problemy medicinskoj mikologii, 2, 63. [in Russian].

3. Gelfond, B. R., Gologorskij, V. A. & Gelfond, E. B. (2002). Kandidoznaya infekciya v hirurgii i intensivnoj terapii. Klinicheskaya mikrobiologiya i antimikrobnaya himioterapiya, 2, 23–30. [in Russian].

4. Elinov, N. P. (2007). Candida species i kandidemiya, sostoyanie problemy. Problemy medicinskoj mikologii. Vzyato s [in Russian].

5. Klyasova, G. A. (2004). Kandidoznye infekcii v hirurgicheskoj praktike. Russkij medicinskij zhurnal, 8, 548–551. [in Russian].

6. Milihina, A. V. (1987). Razrabotka i eksperimentalnoe izuchenie suhih pitatelnyh sred dlya diagnostiki kandidoza. [in Russian].

7. Sergeev, A. Yu. & Sergeev, Yu. V. (2000). Kandidoz. Priroda infekcii, mehanizmy agressii i zashity, laboratornaya diagnostika i lechenie: rukovodstvo dlya vrachej. Moskva: Triada-H. [in Russian].

8. Binelli, C. F. Moretti, M. L. & Assis, R. S. (2006). Investigation of the possible association between nosocomial candiduria and candidaemia. Clin. Microbiol. Infect., 12 (6), 538–543.

9. Kauffmann, C. A., Vazquez, J. A. & Sobel, J. D. (2000). Prospective Multicenter Surveillance Study of Funguria in Hospitalized Patiens. Clin. Inf. Dis., 30, 14–18.

10. Viscoli, C., Girmenia, C. & Marinus, A. (1995). Surveillance study of fungemia in cancer patients in Europe. Invasive Fungal Infections Cooperative Group (IFIG of EORTC). Trends in Invasive Fungal Infections, 3. Brussels.
How to Cite
SоbkоvаJ., Frantsishko, A., Filonenko, G., Rosada, M., & Mihienkova, A. (2017). Development and use of the modified Saburo medium for the isolation of Candida strains from biological material from patients. Reports of Vinnytsia National Medical University, 21(1(2), 323-326. Retrieved from