Development and use of the modified Saburo medium for the isolation of Candida strains from biological material from patients
Keywords:
candidiasis, fungi Candida species, isolate, Sabouraud medium.
Abstract
In the detection of clinically important yeasts, the traditional use of Sabouraud medium for microbial isolation from the clinical specimens from the wards patients of multidisciplinary hospital often leads to false-negative results. There was a 10.8%-increase of yeast isolates if we used the complemented with yeast extract modified Sabouraud medium compared to classic Sabouraud medium. The use of modified Sabouraud medium could also significantly reduce time (1-2 days) required for the process of yeasts identification.
References
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3. Gelfond, B. R., Gologorskij, V. A. & Gelfond, E. B. (2002). Kandidoznaya infekciya v hirurgii i intensivnoj terapii. Klinicheskaya mikrobiologiya i antimikrobnaya himioterapiya, 2, 23–30. [in Russian].
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5. Klyasova, G. A. (2004). Kandidoznye infekcii v hirurgicheskoj praktike. Russkij medicinskij zhurnal, 8, 548–551. [in Russian].
6. Milihina, A. V. (1987). Razrabotka i eksperimentalnoe izuchenie suhih pitatelnyh sred dlya diagnostiki kandidoza. [in Russian].
7. Sergeev, A. Yu. & Sergeev, Yu. V. (2000). Kandidoz. Priroda infekcii, mehanizmy agressii i zashity, laboratornaya diagnostika i lechenie: rukovodstvo dlya vrachej. Moskva: Triada-H. [in Russian].
8. Binelli, C. F. Moretti, M. L. & Assis, R. S. (2006). Investigation of the possible association between nosocomial candiduria and candidaemia. Clin. Microbiol. Infect., 12 (6), 538–543.
9. Kauffmann, C. A., Vazquez, J. A. & Sobel, J. D. (2000). Prospective Multicenter Surveillance Study of Funguria in Hospitalized Patiens. Clin. Inf. Dis., 30, 14–18.
10. Viscoli, C., Girmenia, C. & Marinus, A. (1995). Surveillance study of fungemia in cancer patients in Europe. Invasive Fungal Infections Cooperative Group (IFIG of EORTC). Trends in Invasive Fungal Infections, 3. Brussels.
Published
2017-09-29
How to Cite
SоbkоvаJ., Frantsishko, A., Filonenko, G., Rosada, M., & Mihienkova, A. (2017). Development and use of the modified Saburo medium for the isolation of Candida strains from biological material from patients. Reports of Vinnytsia National Medical University, 21(1(2), 323-326. Retrieved from https://reports-vnmedical.com.ua/index.php/journal/article/view/143
Section
METHODS
