Mechanisms of mitochondrial apoptosis in combined trauma of the chest and hips and justification of the usage of antioxidants in experiment


  • M.I. Marushchak
  • M.M. Khudobiak
  • G.G. Gabor
  • O.P. Mialiuk
Keywords: combined trauma, free oxygen radicals, transmembrane potential, apoptosis, correction.

Abstract

The aim of our study was to determine the features of apoptosis when there is a combined trauma of the chest and hips and substantiate the efficacy of antioxidants in this condition. Statistically significant hyperproduction of reactive oxygen leukocytes within 1 day of the experiment, which causes disruption of mitochondrial membrane by bioelectrical activity, characterized by dynamic growth in the percentage of leukocyte cell suspensions with low transmembrane potential of a maximum of 7 days (2.6 times higher than control rate, р≤0.001) in posttraumatic period after undergoing combined trauma of the chest and both thighs. It was also dynamic, statistically significant increase in the percentage of white blood cells with signs of apoptosis since day 1 of the experiment with the highest values in the period of 7-14 days. Statistically significant average relationship between reactive oxygen and severity of apoptosis in the first 7 days and 28 days (r≤0,05) and true interdependence between the level of the transmembrane potential and apoptosis through 3 (rxy=0.63) and 7 days (rxy=0.70).

The usage of antioxidants in posttraumatic period of combined trauma of the chest and both thighs have the positive effect of decreased percentage of free oxygen radicals (Emoxipin action is evident after 7 and 28 days, Mexicor – starting from 3 days with a maximum action after 7 days). The percentage of cells with low leukocyte suspension transmembrane potential and FITC Annexin V-positive cells leukocyte reduced suspension of the application of the two adjustment factors, but a positive impact Mexicor respect Emoxipin is significantly higher.

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Published
2017-09-29
How to Cite
Marushchak, M., Khudobiak, M., Gabor, G., & Mialiuk, O. (2017). Mechanisms of mitochondrial apoptosis in combined trauma of the chest and hips and justification of the usage of antioxidants in experiment. Reports of Vinnytsia National Medical University, 21(1(2), 204-210. Retrieved from https://reports-vnmedical.com.ua/index.php/journal/article/view/109